Herein, a novel fluorescent probe, GTP, was developed for monitoring the GGT (γ-glutamyl transpeptidase) level in living cells and biopsies. It consisted of the typical recognition group γ-Glu (γ-Glutamylcysteine) and the fluorophore (E)-4-(4-aminostyryl)-1-methylpyridin-1-ium iodide. With a ratio response between the signal intensity at 560 nm and 500 nm (R_I560/_I500), it could be important complement for the turn-on ones. With the linear range of 0–50 U/L, the limit of detection was calculated as 0.23 μM. The detection system showed the strongest response near pH 7.4, and exhibited steady fluorescence signals for at least 48 h. With high selectivity, good anti-interference and low cytotoxicity, GTP was suitable for physiological applications. By monitoring the GGT level with the ratio values in the green and blue channels, the probe GTP could distinguish cancer cells from normal cells. Furthermore, in the mouse tissues and humanization tissue samples, the probe GTP could also recognize the tumor tissues from the normal ones.

在此，开发了一种新型荧光探针GTP，用于监测活细胞和活组织检查中的 GGT（γ-谷氨酰转肽酶）水平。它由典型的识别基团γ -Glu ( γ -Glutamylcysteine) 和荧光团 ( E )-4-(4-aminostyryl)-1-methylpyridin-1-ium iodide 组成。560 nm 和 500 nm 信号强度之间的比率响应 (R _I560 / _I500), 它可能是对开启的重要补充。线性范围为 0–50 U/L，检测限计算为 0.23 μM。检测系统在 pH 7.4 附近表现出最强的响应，并且在至少 48 小时内表现出稳定的荧光信号。GTP具有高选择性、良好的抗干扰性和低细胞毒性，适合生理学应用。通过用绿色和蓝色通道中的比率值监测 GGT 水平，探针GTP可以区分癌细胞和正常细胞。此外，在小鼠组织和人源化组织样本中，GTP探针也可以从正常组织中识别出肿瘤组织。