用于复杂样品高灵敏度分析的按需便携式纸基电喷雾电离质谱仪,Analytical Chemistry

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用于复杂样品高灵敏度分析的按需便携式纸基电喷雾电离质谱仪
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-30 , DOI: 10.1021/acs.analchem.3c00673
Yajun Zheng ₁ , Yajie Huang ₁ , Qianqian Zuo ₁ , Yan Zhang ₁ , Yuhua Wu ₁ , Zhiping Zhang ₁

Affiliation

纸喷雾电离已被证明是最有前途的基于底物的来源，但该技术存在目标化合物解吸效率低和便携性差的问题。在当前的研究中，我们描述了一种便携式纸基电喷雾电离 (PPESI)，其中将一张三角形纸和吸附剂依次装入改进的一次性微量移液器吸头中。该源不仅捕捉了纸喷雾和吸附剂的特点，可高效抑制样品基质以进行目标化合物分析，而且还利用微量移液器吸头来防止喷雾溶剂快速蒸发。开发的 PPESI 的性能取决于填充吸附剂、纸质基材和喷雾溶剂的类型和数量以及施加的电压。此外，与其他相关来源相比，PPESI 与 MS 串联的分析灵敏度和喷雾持续时间分别提高了 2.8-32.3 和 2.0-13.3 倍。基于其高准确度 (>96%) 和精密度（相对标准偏差小于 3%），PPESI 与质谱仪联用已被用于测定复杂生物（例如，全血、血清、和尿液）和食物（如牛奶和橙汁）基质，检测限和定量限为 2–4 pg·mL^{分别为–1}和 7–13 pg mL ^–1。考虑到便携性、高灵敏度和可重复性，该技术可能是复杂样品分析的有前途的替代方案。

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On-Demand Portable Paper-Based Electrospray Ionization Mass Spectrometry for High-Sensitivity Analysis of Complex Samples

Paper spray ionization has been demonstrated to be the most promising substrate-based source, but this technique suffers from the low desorption efficiency of target compounds and poor portability. In the current study, we describe a portable paper-based electrospray ionization (PPESI) in which a piece of triangle paper and adsorbent are packed sequentially into a modified disposable micropipette tip. This source not only captures the feature of paper spray and adsorbent for highly efficient suppression of sample matrixes for target compound analysis but also takes advantage of a micropipette tip to prevent spray solvent from rapid evaporation. The performance of developed PPESI depends on the type and amount of packed adsorbent, paper substrate, and spray solvent and applied voltage. Moreover, by contrast to other related sources, the analytical sensitivity and the spray duration of PPESI in tandem with MS have been improved by factors of 2.8–32.3 and 2.0–13.3, respectively. Based on its high accuracy (>96%) and precision (less than 3% relative standard deviation), the PPESI coupled to a mass spectrometer has been used to determine diverse therapeutic drugs and pesticides in complex biological (e.g., whole blood, serum, and urine) and food (e.g., milk and orange juice) matrixes, and the limits of detection and quantification were 2–4 pg mL^–1 and 7–13 pg mL^–1, respectively. Taking the portability, high sensitivity, and repeatability, the technique may be a promising alternative for complex sample analysis.

更新日期：2023-03-30

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