Abstract

The widespread use of human MSCs in biomedical technologies and the important role of cell migration in biomedical processes lead to the need to deepen fundamental researches of these cells’ motility during replicative senescence (RS). In this work, we performed a comparative analysis of the structure of the actin cytoskeleton and characteristics of the DF-2 cells’ motility with the presence of an activator (LPA) or an inhibitor (Y-27632) of the small GTPase RhoA at different stages of RS. The following results were obtained: 1) it was shown the presence of RS during long-term cultivation (8–28 passages) of control cells; 2) at the late stage of RS, the presence of 10 ng/mL LPA for 24 h did not cause changes in the structure of the actin cytoskeleton, while the inhibitor of the small GTPase RhoA activity contributed to a significant decrease in the number of stress fibers; 3) changes in the speed and sinuosity of cell movements in the presence of LPA or Y-27632 depend on the stage of RS: in particular, LPA reduces, and Y-27632 increases the mean speed of cell movement at passages 21 and 28 compared with the control, and at passage 8 these agents do not affect cell speed. The results seem to indicate an increase in the activity of RhoA-associated signaling pathways during RS.