用于近红外荧光生物成像的自组装 BODIPY 纳米粒子,Molecules

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用于近红外荧光生物成像的自组装 BODIPY 纳米粒子
Molecules ( IF 4.2 ) Pub Date : 2023-03-28 , DOI: 10.3390/molecules28072997
Jiale Wang ₁ , Zhao Jiang ₁ , Cheng Huang ₂ , Shimao Zhao ₁ , Senqiang Zhu ₁ , Rui Liu ₁ , Hongjun Zhu ₁

Affiliation

活体光学成像在疾病诊断中具有重要的应用价值。然而，具有优异发光性能的近红外纳米探针仍然很少。在此，设计并合成了两种硼-二吡咯甲烯 (BODIPY) 分子（BDP-A 和 BDP-B）。通过调节其核心结构上取代基的给电子能力，将 BODIPY 发射调谐到近红外 (NIR) 区域。此外，在 BODIPY 上引入聚乙二醇 (PEG) 链能够形成自组装纳米粒子 (NP)，从而形成光学纳米探针。自组装的 BODIPY NPs 具有几个优点，包括近红外发射、大斯托克斯位移和高荧光量子效率，可提高水分散性和信噪比，减少生物背景荧光的干扰。体外研究表明，这些 NPs 可以进入肿瘤细胞并通过荧光成像照亮细胞质。然后，由于其独特的 NIR 发射，BDP-B NPs 被选择用于体内成像。BDP-B 在肿瘤中富集，并在注射到小鼠尾静脉后通过增强的穿透性和保留效应 (EPR) 有效地照亮它。有机纳米颗粒通过肝脏和肾脏代谢。因此，具有近红外荧光发射的基于BODIPY的纳米胶束为体内光学纳米探针的开发提供了有效的研究基础。体外研究表明，这些 NPs 可以进入肿瘤细胞并通过荧光成像照亮细胞质。然后，由于其独特的 NIR 发射，BDP-B NPs 被选择用于体内成像。BDP-B 在肿瘤中富集，并在注射到小鼠尾静脉后通过增强的穿透性和保留效应 (EPR) 有效地照亮它。有机纳米颗粒通过肝脏和肾脏代谢。因此，具有近红外荧光发射的基于BODIPY的纳米胶束为体内光学纳米探针的开发提供了有效的研究基础。体外研究表明，这些 NPs 可以进入肿瘤细胞并通过荧光成像照亮细胞质。然后，由于其独特的 NIR 发射，BDP-B NPs 被选择用于体内成像。BDP-B 在肿瘤中富集，并在注射到小鼠尾静脉后通过增强的穿透性和保留效应 (EPR) 有效地照亮它。有机纳米颗粒通过肝脏和肾脏代谢。因此，具有近红外荧光发射的基于BODIPY的纳米胶束为体内光学纳米探针的开发提供了有效的研究基础。BDP-B 在肿瘤中富集，并在注射到小鼠尾静脉后通过增强的穿透性和保留效应 (EPR) 有效地照亮它。有机纳米颗粒通过肝脏和肾脏代谢。因此，具有近红外荧光发射的基于BODIPY的纳米胶束为体内光学纳米探针的开发提供了有效的研究基础。BDP-B 在肿瘤中富集，并在注射到小鼠尾静脉后通过增强的穿透性和保留效应 (EPR) 有效地照亮它。有机纳米颗粒通过肝脏和肾脏代谢。因此，具有近红外荧光发射的基于BODIPY的纳米胶束为体内光学纳米探针的开发提供了有效的研究基础。

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Self-Assembled BODIPY Nanoparticles for Near-Infrared Fluorescence Bioimaging

In vivo optical imaging is an important application value in disease diagnosis. However, near-infrared nanoprobes with excellent luminescent properties are still scarce. Herein, two boron–dipyrromethene (BODIPY) molecules (BDP-A and BDP-B) were designed and synthesized. The BODIPY emission was tuned to the near-infrared (NIR) region by regulating the electron-donating ability of the substituents on its core structure. In addition, the introduction of polyethylene glycol (PEG) chains on BODIPY enabled the formation of self-assembled nanoparticles (NPs) to form optical nanoprobes. The self-assembled BODIPY NPs present several advantages, including NIR emission, large Stokes shifts, and high fluorescence quantum efficiency, which can increase water dispersibility and signal-to-noise ratio to decrease the interference by the biological background fluorescence. The in vitro studies revealed that these NPs can enter tumor cells and illuminate the cytoplasm through fluorescence imaging. Then, BDP-B NPs were selected for use in vivo imaging due to their unique NIR emission. BDP-B was enriched in the tumor and effectively illuminated it via an enhanced penetrability and retention effect (EPR) after being injected into the tail vein of mice. The organic nanoparticles were metabolized through the liver and kidney. Thus, the BODIPY-based nanomicelles with NIR fluorescence emission provide an effective research basis for the development of optical nanoprobes in vivo.

更新日期：2023-03-29

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