Polysaccharides are the essential active constituents of Radix Pueraria lobata. Numerous studies have shown that polysaccharides can play important roles in ulcerative colitis (UC). This study aimed to determine the protective effects of Radix Pueraria lobata polysaccharide (PPL) against UC. The pharmacological effects of PPL on the dextran sulfate sodium (DSS)-induced UC mice, changes in gut microbiota and metabolism, and DSS-induced Caco-2 cell injury were assessed in this study. Both the PPL inhibited the DSS-induced inflammation both in vivo and in vitro. Mechanistically, PPL inhibited inflammation by decreasing the activation of p-PI3K. Knocking down the PI3K gene resulted in abolishing the inhibitory effects of PPL on mouses and Caco-2 cells. The study concluded that the effects of PPL were completely dependent on PI3K.

多糖是葛根的主要活性成分。大量研究表明，多糖可以在溃疡性结肠炎 (UC) 中发挥重要作用。本研究旨在确定葛根多糖(PPL) 对 UC的保护作用。本研究评估了 PPL 对葡聚糖硫酸钠 (DSS) 诱导的 UC 小鼠的药理作用、肠道微生物群和代谢的变化以及 DSS 诱导的 Caco-2 细胞损伤。PPL在体内和体外均抑制 DSS 诱导的炎症。从机制上讲，PPL 通过降低 p-PI3K 的激活来抑制炎症。敲除PI3K基因导致消除 PPL 对小鼠和 Caco-2 细胞的抑制作用。该研究得出结论，PPL 的作用完全取决于 PI3K。