收缩发夹 DNA 介导的双模式策略通过表面增强拉曼散射和荧光分析同时定量乳铁蛋白和铁离子,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

收缩发夹 DNA 介导的双模式策略通过表面增强拉曼散射和荧光分析同时定量乳铁蛋白和铁离子
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-27 , DOI: 10.1021/acs.analchem.2c05473
Lu Huang ₁ , Hanbing Huang ₁ , Zhuomin Zhang ₁ , Gongke Li ₁

Affiliation

DNA介导的自组装技术具有良好的灵敏度和亲和能力，在探针传感领域得到迅速发展。通过探针传感方法高效准确地定量测定人血清和牛奶样品中的乳铁蛋白（Lac）和铁离子（Fe ^{3+ ），可为人类健康和贫血的早期诊断提供有用的线索。}本文制备了收缩发夹DNA介导的Fe ₃ O ₄ /Ag-ZIF8/石墨量子点（Fe ₃ O ₄ /Ag-ZIF8/GQD）NP双模探针，实现了Lac的表面同时定量-增强拉曼散射 (SERS) 和 Fe ³⁺通过荧光 (FL)。在目标存在的情况下，这些双模探针会被适体识别触发并释放 GQD 以产生 FL 响应。_{同时，互补DNA开始收缩，在Fe 3} O ₄ /Ag表面形成新的发夹结构，产生热点，产生良好的SERS响应。因此，由于双模可切换信号在 SERS 模式下从“关”到“开”和在 FL 模式下从“开”到“关”，所提出的双模分析策略具有出色的选择性、灵敏度和准确性。在优化条件下，Lac 在 0.5–100.0 μg/L 范围内和 Fe ^{3+在 0.01–5.0 μmol/L 范围内获得了良好的线性范围}检出限分别为 0.14 μg/L 和 3.8 nmol/L。最后，收缩发夹DNA介导的SERS-FL双模探针成功应用于人血清和牛奶样品中铁离子和Lac的同时定量。

"点击查看英文标题和摘要"

Contractile Hairpin DNA-Mediated Dual-Mode Strategy for Simultaneous Quantification of Lactoferrin and Iron Ion by Surface-Enhanced Raman Scattering and Fluorescence Analysis

DNA-mediated self-assembly technology with good sensitivity and affinity ability has been rapidly developed in the field of probe sensing. The efficient and accurate quantification of lactoferrin (Lac) and iron ions (Fe³⁺) in human serum and milk samples by the probe sensing method can provide useful clues for human health and early diagnosis of anemia. In this paper, contractile hairpin DNA-mediated dual-mode probes of Fe₃O₄/Ag-ZIF8/graphitic quantum dot (Fe₃O₄/Ag-ZIF8/GQD) NPs were prepared to realize the simultaneous quantification of Lac by surface-enhanced Raman scattering (SERS) and Fe³⁺ by fluorescence (FL). In the presence of targets, these dual-mode probes would be triggered by the recognition of aptamer and release GQDs to produce FL response. Meanwhile, the complementary DNA began to shrink and form a new hairpin structure on the surface of Fe₃O₄/Ag, which produced hot spots and generated a good SERS response. Thus, the proposed dual-mode analytical strategy possessed excellent selectivity, sensitivity, and accuracy due to the dual-mode switchable signals from “off” to “on” in SERS mode and from “on” to “off” in FL mode. Under the optimized conditions, a good linear range was obtained in the range of 0.5–100.0 μg/L for Lac and 0.01–5.0 μmol/L for Fe³⁺ and with detection limits of 0.14 μg/L and 3.8 nmol/L, respectively. Finally, the contractile hairpin DNA-mediated SERS-FL dual-mode probes were successfully applied in the simultaneous quantification of iron ion and Lac in human serum and milk samples.

更新日期：2023-03-27

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南