Microchimica Acta ( IF 5.3 ) Pub Date : 2023-03-27 , DOI: 10.1007/s00604-023-05750-x Linjie Wang 1 , Shujun Zheng 1 , Yixin Chen 1 , Caolong Li 1, 2 , Fei Wang 1, 2
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Abstract
A fluorescence and colorimetric tandem dual-mode sensor was established by modulating fluorescence and oxidase-like activity via valence switching of cerium-based coordination polymer nanoparticles (Ce-CPNs) for the detection of sarcosine (Sar) which is considered as a potential biomarker for the diagnosis of prostate cancer (PCa). In the present research, sarcosine oxidase (SOX) specifically catalyzes the oxidation of Sar to yield H2O2 which can rapidly oxidize Ce(III)-CPNs to generate Ce(IV)-CPNs in appropriate alkaline solution. The generated Ce(IV)-CPNs create a markedly weakened fluorescent signal at 350 nm, while they can induce oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate blue TMBox through emerging good oxidase-like activity. The sensing platform can realize accurate, stable, and high-throughput detection of Sar because of the tandem dual signal output mechanism. More attractively, the chromogenic hydrogel sensing device using smartphone photographing has achieved perfect results for the on-site sensing of Sar in urine specimens without large experimental equipments, demonstrating its considerable clinical application potential in the early diagnosis of PCa.
Graphical abstract
中文翻译：
通过铈基配位聚合物纳米粒子的价态转换调节荧光和类氧化酶活性构建荧光和比色串联双模传感器用于肌氨酸检测
摘要
通过铈基配位聚合物纳米粒子 (Ce-CPNs) 的价态转换调节荧光和氧化酶样活性，建立了荧光和比色串联双模式传感器，用于检测被认为是潜在生物标志物的肌氨酸 (Sar)前列腺癌 (PCa) 的诊断。在目前的研究中，肌氨酸氧化酶 (SOX) 特异性催化 Sar 氧化生成 H 2 O 2它可以在适当的碱性溶液中快速氧化 Ce(III)-CPNs 生成 Ce(IV)-CPNs。生成的 Ce(IV)-CPNs 在 350 nm 处产生明显减弱的荧光信号，同时它们可以通过新出现的良好氧化酶样活性诱导 3,3',5,5'-四甲基联苯胺 (TMB) 氧化生成蓝色 TMBox。由于串联双信号输出机制，传感平台可以实现对Sar的准确、稳定、高通量检测。更吸引人的是，利用智能手机拍照的显色水凝胶传感装置，在无需大型实验设备的情况下，对尿液标本中Sar的现场检测取得了完美的结果，展示了其在PCa早期诊断中的巨大临床应用潜力。
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