Applied Biochemistry and Microbiology ( IF 1.0 ) Pub Date : 2023-03-26 , DOI: 10.1134/s0003683823010052
I. S. Nesterenko , O. D. Hendrickson , N. I. Smirnova , S. A. Eremin , D. V. Sotnikov
|
A fluorescence polarization immunoassay has been developed for the detection of the antibacterial sulfathiazole. The latter belongs to the sulfonamide class of antibacterial, which is used in beekeeping for the prevention and treatment of infectious diseases of bees. The assay conditions, which ensure the achievement of high analytical characteristics, are determined. The duration of the analysis is 5 min; the limit of sulfathiazole detection is 3 ng/mL. A study of the assay selectivity concerning other sulfonamides demonstrated high specificity only towards sulfathiazole. An optimal method of sample preparation before control honey contamination was proposed. The effectiveness of the developed analysis for the detection of sulfathiazole in honey samples was confirmed. The developed fluorescent polarization immunoassay is a rapid and efficient analytical system for rapid and sensitive control of honey quality and safety.
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
磺胺噻唑偏振荧光免疫分析法的研制及其在蜂蜜检测中的应用
已经开发了一种荧光偏振免疫测定法来检测抗菌磺胺噻唑。后者属于磺胺类抗菌药,用于养蜂业,用于防治蜜蜂的传染病。确定了确保实现高分析特性的测定条件。分析持续时间为 5 分钟;磺胺噻唑的检测限为 3 ng/mL。一项关于其他磺胺类药物的测定选择性研究表明,仅对磺胺噻唑具有高特异性。提出了控制蜂蜜污染前样品制备的最佳方法。证实了所开发的用于检测蜂蜜样品中磺胺噻唑的分析方法的有效性。