Construction of a water-soluble fluorescent probe for copper (II) ion detection in live cells and food products,Food Chemistry

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Construction of a water-soluble fluorescent probe for copper (II) ion detection in live cells and food products
Food Chemistry ( IF 8.5 ) Pub Date : 2023-03-23 , DOI: 10.1016/j.foodchem.2023.135994
Hui Sun ₁ , Qingyu Xu ₁ , Chen Xu ₁ , Yukun Zhang ₁ , Jindong Ai ₁ , Mingguang Ren ₁ , Keyin Liu ₁ , Fangong Kong ₁

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The quality of wine can be affected by excess Cu2+ due to the occurrence of oxidation reactions or precipitation. Therefore, it is essential to use simple and effective testing methods to ensure the Cu2+ content in wine. In this work, we designed and synthesized a rhodamine polymer fluorescent probe (PEG-R). The water solubility of PEG-R was improved by the introduction of polyethylene glycol, which improved the performance and broadened its application in the food field. The PEG-R was characterized by high sensitivity, selectivity and fast response to Cu2+ and was able to complete the response process within 30 s, with approximately 29-fold fluorescence enhancement of the probe after exposure to Cu2+, the limit of detection (LOD) was 1.295 × 10-6 M. The probe can be used for the determination of Cu2+ in living cells, zebrafish, white wine and food products, and it was made into practical gels and test strips.

中文翻译：

用于活细胞和食品中铜（II）离子检测的水溶性荧光探针的构建

由于发生氧化反应或沉淀，过量的 Cu2+ 会影响葡萄酒的质量。因此，必须使用简单有效的检测方法来确保葡萄酒中的 Cu2+ 含量。在这项工作中，我们设计并合成了罗丹明聚合物荧光探针（PEG-R）。通过引入聚乙二醇，提高了 PEG-R 的水溶性，从而提高了性能并拓宽了其在食品领域的应用。PEG-R 具有高灵敏度、选择性和对 Cu2+ 的快速响应，能够在 30 s 内完成响应过程，暴露于 Cu2+ 后探针荧光增强约 29 倍，检测限（LOD）为 1.295 × 10-6 M。该探针可用于测定活细胞、斑马鱼、白葡萄酒和食品中的 Cu2+，并被制成实用的凝胶和试纸。

更新日期：2023-03-23

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