在高温下询问金属有机框架内的封装蛋白质结构,Journal of the American Chemical Society

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在高温下询问金属有机框架内的封装蛋白质结构
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2023-03-24 , DOI: 10.1021/jacs.2c13525
Rohan Murty ₁ , Mrinal K Bera ₂ , Ian M Walton ₁ , Christina Whetzel ₁ , Mark R Prausnitz ₁ , Krista S Walton ₁

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将生物大分子封装在金属有机框架 (MOF) 中可以赋予被困客体热稳定性。据推测，将客体分子限制在刚性 MOF 支架内会提高客体的稳定性，但没有直接证据表明这种机制。在这里，我们提出了一种新的分析方法，使用小角度 X 射线散射 (SAXS) 来解析牛血清白蛋白 (BSA) 的结构，同时封装在两个沸石咪唑酯框架（ZIF-67 和 ZIF-8）中。我们的方法包括从生物复合材料 BSA@ZIF 中减去 ZIF 的缩放 SAXS 光谱，通过 Guinier、Kratky 和对距离分布函数分析来确定封装 BSA 的回转半径。当暴露于 70 °C 的原生 BSA 变性时，原位 SAXS 分析表明，当封装在 ZIF 支架内时，封装的 BSA 在 70 °C 时保持其大小和折叠状态，这表明 MOF 腔内的截留抑制了蛋白质展开，从而抑制了蛋白质变性。这种 SAXS 分析方法不仅可以深入了解 MOF 中的生物分子稳定性，还可以提供一种新方法来研究刚性基质中其他构象不稳定分子的结构。

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Interrogating Encapsulated Protein Structure within Metal–Organic Frameworks at Elevated Temperature

Encapsulating biomacromolecules within metal–organic frameworks (MOFs) can confer thermostability to entrapped guests. It has been hypothesized that the confinement of guest molecules within a rigid MOF scaffold results in heightened stability of the guests, but no direct evidence of this mechanism has been shown. Here, we present a novel analytical method using small-angle X-ray scattering (SAXS) to solve the structure of bovine serum albumin (BSA) while encapsulated within two zeolitic imidazolate frameworks (ZIF-67 and ZIF-8). Our approach comprises subtracting the scaled SAXS spectrum of the ZIF from that of the biocomposite BSA@ZIF to determine the radius of gyration of encapsulated BSA through Guinier, Kratky, and pair distance distribution function analyses. While native BSA exposed to 70 °C became denatured, in situ SAXS analysis showed that encapsulated BSA retained its size and folded state at 70 °C when encapsulated within a ZIF scaffold, suggesting that entrapment within MOF cavities inhibited protein unfolding and thus denaturation. This method of SAXS analysis not only provides insight into biomolecular stabilization in MOFs but may also offer a new approach to study the structure of other conformationally labile molecules in rigid matrices.

更新日期：2023-03-24

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