The effects of konjac glucomannan (KGM) with different deacetylation degrees (DDs) on the gel properties of whey protein isolate (WPI) were investigated. The appropriately deacetylated KGM (DDs in the range of 0–53.85 %) incorporated within WPI and formed relatively uniform compound gels, while excessive deacetylated KGM (DDs = 63.46 or 71.63 %) caused macroscopic precipitation and aggregation in WPI-KGM system. The water holding capacity of WPI-KGM gels decreased with the gradual increase of DDs, and the removal of acetyl groups reduced the whiteness of the composite gels. The hardness and chewiness of the composite gel tended to increase and subsequently decrease with the enhancement of DDs, and reached the maximum (244.15 and 148.88 g, respectively) at the DDs of 53.85 %. The rheological analysis indicated that rigid structured WPI-KGM gels could be formed when incorporated with moderately deacetylated KGM. The deacetylated KGM (DDs = 53.85 %) enhanced the hydrogen bond and disulfide bond within the mixed system, resulting in a more compact network structure of the composite gels. Moreover, deacetylated KGM particles might also reinforce the gel strength by the “filling effects”. Overall, the gelation characteristics of the WPI-KGM system can be regulated by controlling the DDs of KGM.