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Mitochondrial Membrane Potential Independent Near-Infrared Mitochondrial Viscosity Probes for Real-Time Tracking Mitophagy
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-20 , DOI: 10.1021/acs.analchem.2c05568 Jiaxin Hong 1 , Xiaogang Guan 1 , Yao Chen 1 , Xiaodong Tan 1 , Shiya Zhang 1 , Guoqiang Feng 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-20 , DOI: 10.1021/acs.analchem.2c05568 Jiaxin Hong 1 , Xiaogang Guan 1 , Yao Chen 1 , Xiaodong Tan 1 , Shiya Zhang 1 , Guoqiang Feng 1
Affiliation
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Mitophagy is a vital cellular process playing vital roles in regulating cellular metabolism and mitochondrial quality control. Mitochondrial viscosity is a key microenvironmental index, closely associated with mitochondrial status. To monitor mitophagy and mitochondrial viscosity, three molecular rotors (Mito-1, Mito-2, and Mito-3) were developed. All probes contain a cationic quinolinium unit and a C12 chain so that they can tightly bind mitochondria and are not affected by the mitochondrial membrane potential. Optical studies showed that all probes are sensitive to viscosity changes with an off–on fluorescence response, and Mito-3 shows the best fluorescence enhancement. Bioimaging studies showed that all these probes can not only tightly locate and visualize mitochondria with near-infrared fluorescence but also effectively monitor the mitochondrial viscosity changes in cells. Moreover, Mito-3 was successfully applied to visualize the mitophagy process induced by starvation, and mitochondrial viscosity was found to show an increase during mitophagy. We expect Mito-3 to become a useful imaging tool for studying mitochondrial viscosity and mitophagy.
中文翻译：
用于实时跟踪线粒体自噬的线粒体膜电位独立近红外线粒体粘度探针
线粒体自噬是一个重要的细胞过程，在调节细胞代谢和线粒体质量控制方面发挥着重要作用。线粒体粘度是一个关键的微环境指标，与线粒体状态密切相关。为了监测线粒体自噬和线粒体粘度，开发了三种分子转子（Mito-1、Mito-2和Mito-3 ）。所有探针都含有阳离子喹啉单元和C 12链，因此它们可以紧密结合线粒体并且不受线粒体膜电位的影响。光学研究表明，所有探针都对粘度变化敏感，具有关-开荧光响应，而Mito-3显示出最好的荧光增强。生物成像研究表明，所有这些探针不仅可以通过近红外荧光紧密定位和可视化线粒体，还可以有效监测细胞中线粒体粘度的变化。此外，Mito-3成功应用于可视化由饥饿引起的线粒体自噬过程，并且发现线粒体粘度在线粒体自噬过程中增加。我们期待Mito-3成为研究线粒体粘度和线粒体自噬的有用成像工具。
更新日期：2023-03-20
中文翻译：
用于实时跟踪线粒体自噬的线粒体膜电位独立近红外线粒体粘度探针
线粒体自噬是一个重要的细胞过程，在调节细胞代谢和线粒体质量控制方面发挥着重要作用。线粒体粘度是一个关键的微环境指标，与线粒体状态密切相关。为了监测线粒体自噬和线粒体粘度，开发了三种分子转子（Mito-1、Mito-2和Mito-3 ）。所有探针都含有阳离子喹啉单元和C 12链，因此它们可以紧密结合线粒体并且不受线粒体膜电位的影响。光学研究表明，所有探针都对粘度变化敏感，具有关-开荧光响应，而Mito-3显示出最好的荧光增强。生物成像研究表明，所有这些探针不仅可以通过近红外荧光紧密定位和可视化线粒体，还可以有效监测细胞中线粒体粘度的变化。此外，Mito-3成功应用于可视化由饥饿引起的线粒体自噬过程，并且发现线粒体粘度在线粒体自噬过程中增加。我们期待Mito-3成为研究线粒体粘度和线粒体自噬的有用成像工具。
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