Engineered Extracellular Vesicle-Encapsuled Nanoreactors for Effective Targeting and Cascade Killing of Cancer Cells,ACS Applied Bio Materials

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Engineered Extracellular Vesicle-Encapsuled Nanoreactors for Effective Targeting and Cascade Killing of Cancer Cells
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2023-03-21 , DOI: 10.1021/acsabm.2c01019
Man-Sha Wu ₁ , Ze-Rui Zhou ₁ , Xiao-Yuan Wang ₁ , Jian Lv ₁ , Da-Wei Li ₁ , Ruo-Can Qian ₁

Affiliation

Nanomaterials have presented great potential for cancer therapy. However, their therapeutic efficacy is not always satisfied because of inefficient biocompatibility and targeting efficacy. Here, we report engineered extracellular vesicle (EV)-encapsuled nanoreactors for the targeting and killing of cancer cells. EVs are extracted from engineered cancer cells with surface N-glycans cut and intracellular microRNA-21 (miR-21) silenced to generate cancer-targeting membranes for the following coating of gold–polydopamine (PDA) core–shell nanoparticles. The encapsuled nanoparticles are decorated with doxorubicin (Dox), glucose oxidase (GOx), and miR-21-indicative DNA tags. Once endocytosed, the acidic pH, together with the photothermal effect of the PDA shell, can promote the release of Dox and GOx-catalyzed H₂O₂ generation/glucose consumption, while the DNA tags allow enhanced fluorescence imaging of miR-21 to indicate the targeting effect. The coadministration of EV-assisted delivery and cascade treatment represents a promising strategy for combination therapy.

中文翻译：

用于有效靶向和级联杀伤癌细胞的工程化细胞外囊泡封装纳米反应器

纳米材料在癌症治疗方面具有巨大潜力。然而，由于低效的生物相容性和靶向功效，它们的治疗效果并不总是令人满意。在这里，我们报告了用于靶向和杀死癌细胞的工程化细胞外囊泡 (EV) 封装纳米反应器。EV 是从经过表面N聚糖切割和细胞内 microRNA-21 (miR-21) 沉默的工程化癌细胞中提取的，从而为随后的金-聚多巴胺 (PDA) 核-壳纳米粒子涂层生成癌症靶向膜。封装的纳米粒子装饰有多柔比星 (Dox)、葡萄糖氧化酶 (GOx) 和 miR-21 指示性 DNA 标签。一旦被内吞，酸性 pH 值连同 PDA 壳的光热效应可以促进 Dox 和 GOx 催化的 H₂ O ₂生成/葡萄糖消耗，而 DNA 标签允许增强 miR-21 的荧光成像以指示靶向作用。EV 辅助分娩和级联治疗的共同给药代表了一种有前途的联合治疗策略。

更新日期：2023-03-21

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