Abnormal SO₂ derivatives level induces mitochondrial and lysosomal dysfunction, which is associated with a variety of diseases. Therefore, it is imperative to develop fluorescent probes which are suitable for detection of SO₂ derivatives in biological systems. Herein, we developed two new ratiometric fluorescent probes, MByn and HByn, which can respond to HSO₃⁻ in PBS buffer solution accurately and rapidly on basis of Michael addition reaction. After addition of HSO₃⁻, ratiometric response signal increases by 5 folds, accompanied by a macroscopic change of fluorescence color. What's more, the two novel fluorescent probes MByn and HByn exhibited low detection limit (LOD = 0.96/0.79 μM), good photostability and low cytotoxicity. Cell imaging experiments showed that MByn and HByn were able to localize mitochondria and lysosomes accurately and detect both exogenous and endogenous SO₂ derivatives in living cells.

异常的SO ₂衍生物水平诱导线粒体和溶酶体功能障碍，这与多种疾病相关。因此，开发适用于检测生物体系中SO ₂衍生物的荧光探针势在必行。在此，我们基于迈克尔加成反应开发了两种新型比例荧光探针MByn和HByn，它们可以准确快速地响应PBS缓冲溶液中的HSO ₃ ^{- 。}添加 HSO _3后^-，比率响应信号增加 5 倍，伴随着荧光颜色的宏观变化。此外，这两种新型荧光探针 MByn 和 HByn 具有低检测限（LOD = 0.96/0.79 μM）、良好的光稳定性和低细胞毒性。细胞成像实验表明，MByn 和 HByn 能够准确定位线粒体和溶酶体，并检测活细胞中的外源性和内源性 SO _{2衍生物。}