We report on a noninvasive hypoxia monitor based on a biosensor linearly responding to lactate through its whole concentration range in human sweat. The biosensor is designed with an additional perfluorosulfonated ionomer (Nafion analogue) membrane acting as a diffusion barrier for lactate due to its intrinsic negative charge. The resulting biosensor linearly responds to lactate in the range from 1 to 100 mM and displays significantly improved operational stability, maintaining its initial response during 3 h in human sweat flow. Thus, the elaborated monitor has been validated through on-body continuous sweat analysis during exhaustive physical exercise. In contrast to periodic sweat sampling, the elaborated monitor allows recording of the true extremes of sweat lactate, which are of particular importance for diagnostics.

我们报告了一种基于生物传感器的无创缺氧监测仪，该生物传感器对人体汗液中乳酸的整个浓度范围呈线性响应。该生物传感器设计有额外的全氟磺化离聚物（Nafion 类似物）膜，由于其固有的负电荷，充当乳酸的扩散屏障。由此产生的生物传感器对 1 至 100 mM 范围内的乳酸有线性响应，并显示出显着改善的操作稳定性，在人体汗液流动 3 小时内保持其初始响应。因此，精心制作的监测器已通过在剧烈运动期间进行的身体连续汗液分析得到验证。与周期性的汗液采样相比，精心设计的监测器可以记录汗液乳酸的真实极端值，这对诊断特别重要。