Nobel metals have unique and adjustable plasmonic characteristics, which often enable the synthesis of safer forms of biocompatible nanocomposites. In particular, gold (Au) and silver (Ag)-mediated nanocomposites are preferred in the fields of biomedicine due to their intrinsic therapeutic properties. Here, we present a simple and powerful methodological analysis of synthesising the AgO/Ag/ZnO composite through the microwave hydrothermal method. This work reports a novel method of synthesis, characterization, and assessment of cytotoxicity and antimicrobial properties mediated by the AgO/Ag/ZnO composite. The shape and size distribution of the synthesised AgO/Ag/ZnO composite were visualised by TEM analysis, with a size range of 15–25 nm, XRD and XPS analysis confirmed the co-existence of an AgO/Ag/ZnO heterostructure. ZnO has a low density, which make it suitable for catalytic applications. Furthermore, HR-TEM images revealed the integration of AgO, Ag, and ZnO in the composite form, resulting in increased biocatalytic activity. The negatively charged zeta potential (-26 mV) of AgO/Ag/ZnO enhanced its cellular penetration through the enhanced permeability and retention (EPR) effect. The antibacterial activity revealed a greater inhibition zone in E. coli, S. aureus, and B. subtilis, followed in S. typhi. It is found that AgO/Ag/ZnO is potent in inhibiting C. ablicans than standard. AgO/Ag/ZnO showed potent anticancer activity against HCT-116 cell lines with an IC50 value 16.61 μg/ml. The microwave hydrothermal method is a feasible and eco-friendly method for the synthesis of a novel AgO/Ag/ZnO composite. Also, the synthesised nanoparticles could be used to develop industrial and biomedical applications. This is something that needs to be looked into more in the molecular elucidation of the AgO/Ag/ZnO composite.

诺贝尔金属具有独特且可调节的等离子特性，通常能够合成更安全的生物相容性纳米复合材料。特别是，金 (Au) 和银 (Ag) 介导的纳米复合材料因其内在的治疗特性而在生物医学领域受到青睐。在这里，我们提出了一种通过微波水热法合成 AgO/Ag/ZnO 复合材料的简单而强大的方法学分析。这项工作报告了一种新的合成、表征和评估由 AgO/Ag/ZnO 复合材料介导的细胞毒性和抗菌性能的方法。合成的 AgO/Ag/ZnO 复合材料的形状和尺寸分布通过 TEM 分析可视化，尺寸范围为 15-25 nm，XRD 和 XPS 分析证实了 AgO/Ag/ZnO 异质结构的共存。ZnO密度低，这使其适用于催化应用。此外，HR-TEM 图像显示 AgO、Ag 和 ZnO 以复合形式整合，从而提高了生物催化活性。AgO/Ag/ZnO 带负电荷的 zeta 电位 (-26 mV) 通过增强的渗透性和保留 (EPR) 效应增强了其细胞渗透。抗菌活性显示出更大的抑菌圈大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌，其次是伤寒沙门氏菌。发现 AgO/Ag/ZnO 比标准更有效地抑制C. ablicans。AgO/Ag/ZnO 对 HCT-116 细胞系显示出有效的抗癌活性，IC50 值为 16.61 μg/ml。微波水热法是合成新型 AgO/Ag/ZnO 复合材料的一种可行且环保的方法。此外，合成的纳米粒子可用于开发工业和生物医学应用。这是在 AgO/Ag/ZnO 复合材料的分子解析中需要更多研究的东西。