<br>基于 MOF-核酸相互作用的生物分析 DNA 探针传感行为的洞察,Analytical Chemistry

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基于 MOF-核酸相互作用的生物分析 DNA 探针传感行为的洞察
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-15 , DOI: 10.1021/acs.analchem.3c00832
Dinghui Xiong ₁ , Jie Cheng ₂ , Fengxiang Ai ₁ , Xinyu Wang ₁ , Jiaxuan Xiao ₁ , Fang Zhu ₁ , Kun Zeng ₁ , Kun Wang ₃ , Zhen Zhang ₁

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DNA 探针吸附到纳米材料上是一种很有前景的生物测定策略，通常使用荧光或催化活性来产生信号。尽管很重要，但目前对基于纳米材料-DNA相互作用的传感行为缺乏系统的理解，这极大地限制了合理的方法设计及其后续应用。在此，通过采用多功能金属有机框架（MOF）（FeTCPP⊂UiO-66）作为模型来研究该问题，该模型是通过将血红素样配体 FeTCPP 集成到常用的 MOF（UiO-66）中而合成的。我们的结果表明，吸附在 FeTCPP⊂UiO-66 上的荧光标记 DNA 通过光诱导电子转移、荧光共振能量转移和内部过滤效应被猝灭。在不同的DNA结构中，双链DNA和杂交链式反应产物分别由于解吸和构象变化而很大程度上保留了它们的荧光。此外，ssDNA可以最大程度地抑制FeTCPP⊂UiO-66的过氧化物酶活性，并且这种抑制强烈依赖于链长度，但与碱基组成无关。在此基础上，设计了黄曲霉毒素B1荧光/比色双模态检测方法，取得了令人满意的性能，进一步验证了我们的结果。这项研究为基于 MOF-DNA 相互作用的传感行为提供了一些新的见解，表明合理的生物测定设计及其性能改进的应用前景。

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Insight into the Sensing Behavior of DNA Probes Based on MOF–Nucleic Acid Interaction for Bioanalysis

Adsorption of DNA probes onto nanomaterials is a promising strategy for bioassay establishment typically using fluorescence or catalytic activities to generate signals. Albeit important, there is currently a lack of systematic understanding of the sensing behaviors building on nanomaterial–DNA interactions, which greatly limits the rational method design and their subsequent applications. Herein, the issue was investigated by employing multifunctional metal–organic frameworks (MOFs) (FeTCPP⊂UiO-66) as a model that was synthesized via integrating heme-like ligand FeTCPP into commonly used MOFs (UiO-66). Our results demonstrated that the fluorescently labeled DNA adsorbed onto FeTCPP⊂UiO-66 was quenched through photoinduced electron transfer, fluorescence resonance energy transfer, and the internal filtration effect. Among different DNA structures, double-stranded DNA and hybridization chain reaction products largely retained their fluorescence due to desorption and conformational variation, respectively. In addition, ssDNA could maximally inhibit the peroxidase activity of FeTCPP⊂UiO-66, and this inhibition was strongly dependent on the strand length but independent of base composition. On the basis of these discoveries, a fluorescence/colorimetric dual-modal detection was designed against aflatoxin B1 with satisfactory performances obtained to further verify our results. This study provided some new insights into the sensing behaviors based on MOF–DNA interactions, indicating promising applications for rational bioassay design and its performance improvement.

更新日期：2023-03-15

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