To study the details of the effect of pore size on the performance of grafting-ligand ion exchangers, four diethylaminoethyl-dextran grafted macroporous cellulose microspheres with different pore sizes ranging from 164.57 to 281.96 nm were selected to investigate their adsorption equilibria and kinetics for bovine serum albumin (BSA) and γ-globulin. It was found that for whether adsorption equilibria or uptake kinetics, both the proteins showed increasing-then-decreasing trends with the decreasing pore size. More importantly, for a specific protein, the maximum adsorption capacity and fastest uptake rate were achieved on the same media, indicating the existence of a critical pore size (CPZ), at which the adsorption capacity and uptake kinetic are significantly enhanced synchronously. The CPZs of BSA and γ-globulin were identified to be 216.64 and 266.93 nm, respectively. Experimental results and analyses revealed that the high adsorption capacities at CPZ are attributed to the high utilization of binding sites in pores, and the uptake kinetics are enhanced by the combination of fast pore diffusion and chain delivery effect.

为了详细研究孔径对接枝配体离子交换剂性能的影响，选择了四种孔径在 164.57 至 281.96 nm 之间的二乙氨基乙基-葡聚糖接枝大孔纤维素微球，研究它们对牛血清的吸附平衡和动力学白蛋白 (BSA) 和 γ 球蛋白。结果发现，无论是吸附平衡还是摄取动力学，这两种蛋白质都显示出随着孔径减小而先增加后减少的趋势。更重要的是，对于特定蛋白质，在相同介质上实现了最大吸附容量和最快摄取速率，表明存在临界孔径（CPZ），在该临界孔径下吸附容量和摄取动力学同步显着增强。BSA和γ-球蛋白的CPZ被鉴定为216。分别为 64 和 266.93 纳米。实验结果和分析表明，CPZ 的高吸附容量归因于孔中结合位点的高利用率，快速孔扩散和链传递效应的结合增强了吸收动力学。