皮升液体样品的宽带腔增强紫外-可见吸收光谱,Analyst

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皮升液体样品的宽带腔增强紫外-可见吸收光谱
Analyst ( IF 3.6 ) Pub Date : 2023-03-15 , DOI: 10.1039/d3an00143a
Imogen M Fermor-Worth ₁ , Catalin Chimerel _{1,

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Affiliation

吸收光谱由于其无标记特性而成为一种广泛使用的分析技术，但是它在小液体样品中的应用受到相关的短吸收光程的阻碍，这限制了灵敏度。本文介绍了针对薄液膜优化的超灵敏宽带吸收光谱仪开发的新概念。为了提高吸光度测量的灵敏度，在基于光纤的吸收光谱仪 (CEASpec) 上实施了光学腔。光在感兴趣的样品中循环多次以提高灵敏度。仪器的带宽通过选择形成光腔光谱的介电镜来选择，在本实施中，已设置为 200 nm 宽 (250–450 nm)。光谱仪的传感体积由用于将光传输到样品的光纤的选择来规定，在这个实施中，使用了直径为 400 μm 的光纤，对于 5 μm 的薄膜，传感体积为 630 皮升。厚度。两性霉素 B 是一种在 280-450 nm 光谱范围内的宽光吸收剂，在这里用于证明所提出的腔增强吸收光谱仪的能力。在很宽的波长范围内，已经实现了 200 倍范围内的腔体增强因子，即相对于经典吸收光谱的等效光程增加。在 280-450 nm 光谱范围内的宽光吸收剂，在这里被用来证明所提出的腔增强吸收光谱仪的能力。在很宽的波长范围内，已经实现了 200 倍范围内的腔体增强因子，即相对于经典吸收光谱的等效光程增加。在 280-450 nm 光谱范围内的宽光吸收剂，在这里被用来证明所提出的腔增强吸收光谱仪的能力。在很宽的波长范围内，已经实现了 200 倍范围内的腔体增强因子，即相对于经典吸收光谱的等效光程增加。

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Broadband cavity enhanced UV-VIS absorption spectroscopy for picolitre liquid samples

Absorption spectroscopy is a widely used analytical technique due to its label-free nature, however its application to small liquid samples is hampered by the associated short absorption pathlengths, which limit sensitivity. A novel concept for the development of an ultrasensitive broadband absorption spectrometer optimised for thin liquid films is presented here. To enhance sensitivity of the absorbance measurements an optical cavity is implemented on a fibre-based absorption spectrometer (CEASpec). Light is circulated multiple times through the sample of interest to increase sensitivity. The bandwidth of the instrument is chosen by the choice of the dielectric mirrors forming the optical cavity spectra and, in this implementation, has been set to be 200 nm wide (250–450 nm). The sensing volume of the spectroscope is prescribed by the choice of optical fibres employed to deliver light to the sample, and in this implementation fibres of 400 μm in diameter were employed, giving a sensing volume of 630 picolitres for a thin film of 5 μm in thickness. Amphotericin B, a broad light absorber in the 280–450 nm region of the spectrum, was used here to prove the capabilities of the proposed cavity enhanced absorption spectroscope. Cavity enhancement factors, the equivalent pathlength increase over classical absorption spectroscopy, in the range of 200× have been achieved across a broad wavelength range.

更新日期：2023-03-16

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