New types of fibres like lyocell and seacell have been introduced in the market which is providing unique aesthetic performance and eco values to the melange fabrics. Keeping these considerations in mind, the influence of fibre type, blend ratio, and shade depth on mechanical and physical properties of cotton–bamboo rayon, cotton–lyocell, and cotton–seacell knitted fabrics has been examined. Full factorial design is used to make 18 different samples. Quantitative research was conducted and significant analysis is done by ANOVA. Findings indicate that fibre type and blend ratio significantly impact the dimensional, mechanical, and physical properties, such as stitch density, areal density, thickness, porosity, pore size, bursting strength, and abrasion resistance of cotton blended knitted fabrics. The higher contribution of the cotton leads to the higher areal density and thickness while low pilling and abrasion resistance. Cotton–bamboo rayon melange fabric has the greatest areal density, lowest porosity, lowest bursting strength lowest abrasion, and pilling resistance among the tested samples. Cotton-lyocell blended fabrics show the highest bursting strength and abrasion resistance.