超稳定、可再分散、小且高度有机改性的介孔二氧化硅纳米疗法,Journal of the American Chemical Society

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超稳定、可再分散、小且高度有机改性的介孔二氧化硅纳米疗法
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2011-12-21 , DOI: 10.1021/ja208567v
Yu-Shen Lin ₁ , Nardine Abadeer ₁ , Katie R. Hurley ₁ , Christy L. Haynes ₁

Affiliation

由于在生物介质中存在严重的不可逆聚集，介孔二氧化硅纳米粒子的实际生物医学应用受到粒子分散性和稳定性差的限制。为了解决这个问题，已经合成了具有双有机硅烷（亲水性和疏水性硅烷）表面改性的小尺寸的水热处理介孔二氧化硅纳米粒子。这些高度有机修饰的介孔二氧化硅纳米粒子通过透射电子显微镜、X 射线衍射、N(2) 吸附-解吸、动态光散射、zeta 电位和固态 (29)Si 核磁共振进行表征，并且证明它们非常稳定在生理温度下的模拟体液中。此外，它们可以被干燥成粉末状固体，并且很容易在生物介质中重新分散，保持它们的小尺寸至少 15 天。此外，该制备方法可以扩展到合成可再分散的荧光和磁性介孔二氧化硅纳米粒子。高度稳定和可再分散的介孔二氧化硅纳米粒子在体外细胞试验中表现出最小的毒性。最重要的是，两种类型的阿霉素，水溶性阿霉素和水溶性差的阿霉素，可以负载到这些高度稳定的介孔二氧化硅纳米粒子中，并且这些载药纳米粒子也可以很好地再分散在水溶液中。与游离的水溶性多柔比星相比，用负载有水溶性多柔比星的纳米颗粒处理后发现对宫颈癌 (HeLa) 细胞的细胞毒性增强。这些结果表明高度稳定、可再分散和小的介孔二氧化硅纳米粒子是体内生物医学应用的有前途的试剂。该制备方法可扩展到合成可再分散的荧光和磁性介孔二氧化硅纳米粒子。高度稳定和可再分散的介孔二氧化硅纳米粒子在体外细胞试验中表现出最小的毒性。最重要的是，两种类型的阿霉素，水溶性阿霉素和水溶性差的阿霉素，可以负载到这些高度稳定的介孔二氧化硅纳米粒子中，并且这些载药纳米粒子也可以很好地再分散在水溶液中。与游离的水溶性多柔比星相比，用负载有水溶性多柔比星的纳米颗粒处理后发现对宫颈癌 (HeLa) 细胞的细胞毒性增强。这些结果表明高度稳定、可再分散和小的介孔二氧化硅纳米粒子是体内生物医学应用的有前途的试剂。该制备方法可扩展到合成可再分散的荧光和磁性介孔二氧化硅纳米粒子。高度稳定和可再分散的介孔二氧化硅纳米粒子在体外细胞试验中表现出最小的毒性。最重要的是，两种类型的阿霉素，水溶性阿霉素和水溶性差的阿霉素，可以负载到这些高度稳定的介孔二氧化硅纳米粒子中，并且这些载药纳米粒子也可以很好地再分散在水溶液中。与游离的水溶性多柔比星相比，用负载有水溶性多柔比星的纳米颗粒处理后发现对宫颈癌 (HeLa) 细胞的细胞毒性增强。这些结果表明高度稳定、可再分散和小的介孔二氧化硅纳米粒子是体内生物医学应用的有前途的试剂。

"点击查看英文标题和摘要"

Ultrastable, Redispersible, Small, and Highly Organomodified Mesoporous Silica Nanotherapeutics

Practical biomedical application of mesoporous silica nanoparticles is limited by poor particle dispersity and stability due to serious irreversible aggregation in biological media. To solve this problem, hydrothermally treated mesoporous silica nanoparticles of small size with dual-organosilane (hydrophilic and hydrophobic silane) surface modification have been synthesized. These highly organomodified mesoporous silica nanoparticles were characterized by transmission electron microscopy, X-ray diffraction, N(2) adsorption-desorption, dynamic light scattering, zeta potential, and solid-state (29)Si NMR, and they prove to be very stable in simulated body fluid at physiological temperature. Additionally, they can be dried to a powdered solid and easily redispersed in biological media, maintaining their small size for a period of at least 15 days. Furthermore, this preparation method can be expanded to synthesize redispersible fluorescent and magnetic mesoporous silica nanoparticles. The highly stable and redispersible mesoporous silica NPs show minimal toxicity during in vitro cellular assays. Most importantly, two types of doxorubicin, water-soluble doxorubicin and poorly water-soluble doxorubicin, can be loaded into these highly stable mesoporous silica nanoparticles, and these drug-loaded nanoparticles can also be well-redispersed in aqueous solution. Enhanced cytotoxicity to cervical cancer (HeLa) cells was found upon treatment with water-soluble doxorubicin-loaded nanoparticles compared to free water-soluble doxorubicin. These results suggest that highly stable, redispersible, and small mesoporous silica nanoparticles are promising agents for in vivo biomedical applications.

更新日期：2011-12-21

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