Abstract

There is a correlation between teratozoospermia and production of reactive oxygen species leading to poor assisted reproductive techniques outcomes. This study aimed to examine the effect of plasma-rich in growth factors (PRGF) on teratozoospermic samples. Twenty-five teratozoospermic samples were included in this study. After sperm preparation, it was divided into four groups, including 0 (control), 1, 5, and 10% PRGF. Sperm motility, viability (eosin-nigrosin staining), morphology (Papanicolaou staining), DNA fragmentation (sperm chromatin dispersion test), mitochondrial membrane potential (JC-1 staining by flow cytometry), and lipid peroxidation (measurement of malondialdehyde, MDA) were evaluated before and after 1 h of incubation with or without PRGF. Our results showed that after 1 h of incubation, the addition of 1% PRGF improved sperm progressive motility (47.72 ± 13.76%) compared to the control group (17.36 ± 8.50%) (p < 0.001). Also, 1% PRGF preserved the sperm’s total motility (77.50 ± 13.28% vs. 65.63 ± 19.03%, for 1% PRGF and control, respectively) and viability after incubation. The rate of normal sperm morphology was the same between different groups. Higher mitochondrial membrane potential and lower DNA fragmentation were also observed in sperm treated with different concentrations of PRGF compared to the control group, but the differences were non-significant. The MDA levels were significantly decreased in PRGF-treated groups compared to the control group (0.99 ± 0.62, 0.95 ± 0.33, 0.95 ± 0.79, and 1.49 ± 0.27 for 1% PRGF, 5% PRGF, 10% PRGF and control, respectively). Based on our results, it seems that PRGF incubation can improve sperm parameters and especially decrease the level of malondialdehyde as an indicator of oxidative stress, which is one of the main problems of teratozoospermic samples.

摘要

畸形精子症和活性氧的产生之间存在相关性，导致辅助生殖技术结果不佳。本研究旨在探讨富含生长因子（PRGF）的血浆对畸胎精子样本的影响。本研究包括二十五个畸胎精子样本。精子制备后分为四组，包括0（对照）、1、5、10% PRGF。精子活力、活力（伊红-苯胺黑染色）、形态（巴氏染色）、DNA 碎片（精子染色质分散测试）、线粒体膜电位（流式细胞术 JC-1 染色）和脂质过氧化（丙二醛、MDA 测量）在有或没有 PRGF 的情况下孵育 1 小时之前和之后进行评估。我们的结果表明，经过 1 小时的孵化，p < 0.001）。此外，1% PRGF 保留了精子的总活力（1% PRGF 和对照分别为 77.50 ± 13.28% 与 65.63 ± 19.03%）和孵化后的活力。不同组间精子形态正常率相同。与对照组相比，用不同浓度的 PRGF 处理的精子也观察到更高的线粒体膜电位和更低的 DNA 碎片，但差异不显着。与对照组相比，PRGF 治疗组的 MDA 水平显着降低（1% PRGF、5% PRGF、10% PRGF 和对照组分别为 0.99 ± 0.62、0.95 ± 0.33、0.95 ± 0.79 和 1.49 ± 0.27） 。根据我们的结果，PRGF 孵育似乎可以改善精子参数，尤其是降低作为氧化应激指标的丙二醛水平，