PEDOT：具有高导电性和生物相容性的 PSS 水凝胶用于原位细胞传感,Journal of Materials Chemistry B

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PEDOT：具有高导电性和生物相容性的 PSS 水凝胶用于原位细胞传感
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2023-03-15 , DOI: 10.1039/d3tb00014a
Taotao Yang ₁ , Ming Yang ₁ , Chao Xu ₁ , Kun Yang ₁ , Yuming Su ₁ , Yongqin Ye ₂ , Lingyun Dou ₁ , Qin Yang ₁ , Wenbo Ke ₃ , Bin Wang ₂ , Zhiqiang Luo ₁

Affiliation

导电聚合物水凝胶，尤其是聚（3,4-乙烯二氧噻吩）：聚（苯乙烯磺酸盐）（PEDOT：PSS）水凝胶，由于其高导电性和与组织电耦合以进行传感和刺激的能力，在软生物电子学中显示出巨大的前景。然而，由于现有的制备方法苛刻且使用有毒有害试剂，解决PEDOT:PSS水凝胶生物相容性差的问题具有挑战性。在此，我们报告了以带正电的导电聚合物作为交联剂的 PEDOT:PSS 水凝胶的合成，以及 PEDOT:PSS 水凝胶作为活细胞原位电化学传感器的应用。采用该方法制备的PEDOT:PSS水凝胶在不添加任何有毒有害试剂的情况下电导率可达3265 S m ⁻¹. 在室温下使用 PEDOT:PSS 水溶液和导电聚合物单体的简单混合物的简便合成方法也使 PEDOT:PSS 水凝胶具有可打印性以制造图案化电极。此外，所有提出的 PEDOT:PSS 水凝胶都表现出良好的生物相容性。在 PEDOT:PSS 水凝胶中培养的 PC12 细胞分泌的多巴胺的原位电化学检测表明，我们的 PEDOT:PSS 水凝胶具有高电导率和生物相容性，为将生物传感器集成到 3D 细胞培养中提供了巨大潜力。

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PEDOT:PSS hydrogels with high conductivity and biocompatibility for in situ cell sensing

Conducting polymer hydrogels, especially poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) hydrogels, show great promise in soft bioelectronics due to their high conductivity and the ability of electrical coupling with tissues for sensing and stimulation. However, it is challenging to solve the problem of poor biocompatibility of PEDOT:PSS hydrogels due to the existing harsh preparation methods with the use of toxic and harmful reagents. Herein, we report the synthesis of PEDOT:PSS hydrogels with positively charged conductive polymers as a cross-linker and the application of PEDOT:PSS hydrogels as in situ electrochemical sensors for living cells. The conductivities of PEDOT:PSS hydrogels prepared using this method without any toxic or harmful reagents can reach up to 3265 S m⁻¹. The facile synthesis approach with a simple mixture of PEDOT:PSS aqueous solution and the monomers of conductive polymers at room temperature also enables the printability of PEDOT:PSS hydrogels to fabricate patterned electrodes. Moreover, all the proposed PEDOT:PSS hydrogels demonstrated good biocompatibility. The in situ electrochemical detection of dopamine secreted from PC12 cells cultured within PEDOT:PSS hydrogels suggests that our PEDOT:PSS hydrogels with high conductivity and biocompatibility offer great potential for the integration of biosensors within 3D cell culture.

更新日期：2023-03-15

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