基于免疫磁分离和液滴阵列的癌胚抗原自动检测微流控免疫传感器,Analyst

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基于免疫磁分离和液滴阵列的癌胚抗原自动检测微流控免疫传感器
Analyst ( IF 3.6 ) Pub Date : 2023-03-14 , DOI: 10.1039/d2an01922a
Haoran Hu _{1,

2,

3} , Gaozhe Cai ₂ , Zehang Gao _{2,

4} , Cheng Liang ₅ , Fengna Yang _{1,

3} , Xiaohui Dou _{1,

3} , Chunping Jia ₂ , Jianlong Zhao ₂ , Shilun Feng ₂ , Bei Li _{1,

3}

Affiliation

通过生物标志物诊断癌症对人类健康和生活起着重要作用。然而，目前用于检测癌症生物标志物的实验室技术仍然需要熟练的操作员和相关实验室仪器进行费力且耗时的操作。这项工作提出了一种基于自动免疫磁分离平台和液滴阵列微流控芯片并借助图像分析系统快速灵敏检测癌胚抗原 (CEA) 的比色生物传感器。免疫磁性纳米粒子 (MNP) 用于捕获样品中的 CEA。CEA 检测抗体和辣根过氧化物酶 (HRP) 在聚苯乙烯微球 (PS) 上进行修饰，催化过氧化氢和 3,3',5,5'-四甲基联苯胺 (TMB) 作为信号输出。分析显色反应数据，建立CEA浓度标准曲线。MNPs 在微流控芯片中液滴之间的移动是使用自动可编程磁控制系统实现的。这种比色生物传感器已用于同时检测六个 CEA 样品，范围从 100 pg mL⁻¹至 100 ng mL检测限为 14.347 pg mL⁻¹，遵循线性方程： y = −4.773 ln( x ) + 156.26 ^，相关性R ² = 0.9924，整个工作流程可在80分钟内完成。本文设计的微流控免疫传感器具有低成本、自动化、样品消耗少、通量高等优点，在生物化学领域具有广阔的应用前景。

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A microfluidic immunosensor for automatic detection of carcinoembryonic antigen based on immunomagnetic separation and droplet arrays

Diagnosis of cancer by biomarkers plays an important role in human health and life. However, current laboratory techniques for detecting cancer biomarkers still require laborious and time-consuming operation by skilled operators and associated laboratory instruments. This work presents a colorimetric biosensor for the rapid and sensitive detection of carcinoembryonic antigen (CEA) based on an automated immunomagnetic separation platform and a droplet array microfluidic chip with the aid of an image analysis system. Immunomagnetic nanoparticles (MNPs) were used to capture CEA in the samples. CEA-detecting antibodies and horseradish peroxidase (HRP) were modified on polystyrene microspheres (PS), catalysing hydrogen peroxide and 3,3′,5,5′-tetramethylbenzidine (TMB) as signal outputs. Color reaction data were analyzed to establish a CEA concentration standard curve. The movement of MNPs between droplets in the microfluidic chip is achieved using an automatically programmable magnetic control system. This colorimetric biosensor has been used for the simultaneous detection of six CEA samples ranging from 100 pg mL⁻¹ to 100 ng mL⁻¹ with a detection limit of 14.347 pg mL⁻¹ in 10 min, following the linear equation: y = −4.773 ln(x) + 156.26 with a correlation of R² = 0.9924, and the entire workflow can be completed within 80 minutes. The microfluidic immunosensor designed in this paper has the advantages of low cost, automation, low sample consumption, high throughput, and promising applications in biochemistry.

更新日期：2023-03-15

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