Yoda1 双层膜通过促进骨整合和血管生成加速骨重建,Advanced Healthcare Materials

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Yoda1 双层膜通过促进骨整合和血管生成加速骨重建
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2023-03-13 , DOI: 10.1002/adhm.202203105
Jinghong Yang ₁ , Kaiting Yuan ₁ , Tingting Zhang ₁ , Shiqi Zhou ₁ , Weichang Li ₁ , Zetao Chen ₁ , Yan Wang ₁

Affiliation

引导骨再生膜广泛用于防止成纤维细胞穿透并促进成骨细胞修复骨缺损。然而，目前临床上可用的胶原膜缺乏骨诱导和血管生成能力，骨再生能力有限。据报道，由 Yoda1 激活的机械敏感通道 Piezo1 在成骨和血管生成中发挥着至关重要的作用。然而，仅靠Yoda1的应用来维持这种活性是不可持续的。因此，本研究利用静电纺丝技术制备了负载 Yoda1 的双层膜。其与骨缺损接触的内层由垂直排列的纤维组成，调节细胞的增殖和分化，释放Yoda1，促进骨再生。其与软组织接触的外层通过紫外线交联致密有定向纤维，主要是防止成纤维细胞浸润并抑制免疫反应。此外，加载的 Yoda1 通过Piezo1 / RhoA / Rho 相关的卷曲螺旋蛋白激酶 1/Yes1 相关的转录调节信号通路影响成骨和血管生成。结果表明，Yoda1 双层膜在加速骨再生方面具有高效性和多功能性，表明其作为各种临床问题的新型治疗剂的潜力。

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Accelerated Bone Reconstruction by the Yoda1 Bilayer Membrane via Promotion of Osteointegration and Angiogenesis

Guided bone regeneration membranes are widely used to prevent fibroblast penetration and facilitate bone defect repair by osteoblasts. However, the current clinically available collagen membranes lack bone induction and angiogenic capacities, exhibiting limited bone regeneration. The mechanically sensitive channel, Piezo1, which is activated by Yoda1, has been reported to play crucial roles in osteogenesis and angiogenesis. Nevertheless, the application of Yoda1 alone is unsustainable to maintain this activity. Therefore, this study fabricates a Yoda1-loading bilayer membrane using electrospinning technology. Its inner layer in contact with the bone defect is composed of vertically aligned fibers, which regulate the proliferation and differentiation of cells, release Yoda1, and promote bone regeneration. Its outer layer in contact with the soft tissue is dense with oriented fibers by UV cross-linking, mainly preventing fibroblast infiltration and inhibiting the immune response. Furthermore, the loaded Yoda1 affects osteogenesis and angiogenesis via the Piezo1/RhoA/Rho-associated coiled-coil-containing protein kinase 1/Yes1-associated transcriptional regulator signaling pathway. The results reveal that the Yoda1 bilayer membrane is efficient and versatile in accelerating bone regeneration, suggesting its potential as a novel therapeutic agent for various clinical issues.

更新日期：2023-03-13

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