Fenvalerate has the advantages of a broad insecticidal spectrum, high efficiency, low toxicity and low cost, and it is widely used in agriculture, especially in tea, resulting in the accumulation of fenvalerate residues in tea and the environment, posing a serious threat to human health. Therefore, the timely monitoring of fenvalerate residue dynamics is vital for ensuring the health of humans and the ecological environment, and it is necessary for establishing a fast, reliable, accurate and on-site method for detecting fenvalerate residues. Based on the methods of immunology, biochemistry and molecular biology, mammalian spleen cells, myeloma cells and mice were used as experimental materials to establish a rapid detection method of an enzyme-linked immunosorbent assay to detect the residues of fenvalerate in dark tea. Three cell lines-1B6, 2A11 and 5G2-that can stably secrete fenvalerate antibodies were obtained by McAb technology, and their sensitivities (IC50) were 36.6 ng/mL, 24.3 ng/mL and 21.7 ng/mL, respectively. The cross-reaction rates of the pyrethroid structural analogs were all below 0.6%. Six dark teas were used to detect the practical application of fenvalerate monoclonal antibodies. The sensitivity IC50 of the anti-fenvalerate McAb in PBS with 30% methanol is 29.12 ng/mL. Furthermore, a latex microsphere immunochromatographic test strip with an LOD of 10.0 ng/mL and an LDR of 18.9-357 ng/mL was preliminarily developed. A specific and sensitive monoclonal antibody for fenvalerate was successfully prepared and applied to detect fenvalerate in dark teas (Pu'er tea, Liupao tea, Fu Brick tea, Qingzhuan tea, Enshi dark tea and selenium-enriched Enshi dark tea). A latex microsphere immunochromatographic test strip was developed for the preparation of rapid detection test strips of fenvalerate.

中文翻译：

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六种黑茶中氰戊菊酯单克隆抗体的制备、鉴定及初步应用。

氰戊菊酯具有杀虫谱广、高效、低毒、成本低等优点，广泛应用于农业，尤其是茶叶中，致使氰戊菊酯残留在茶叶和环境中不断积累，对人类健康构成严重威胁。健康。因此，及时监测氰戊菊酯残留动态对保障人类健康和生态环境至关重要，需要建立快速、可靠、准确的现场检测氰戊菊酯残留的方法。以免疫学、生物化学和分子生物学方法为基础，以哺乳动物脾细胞、骨髓瘤细胞和小鼠为实验材料，建立酶联免疫吸附法快速检测黑茶中氰戊菊酯残留量的方法。三种细胞系-1B6，采用McAb技术获得可稳定分泌氰戊菊酯抗体的2A11和5G2-，其敏感性（IC50）分别为36.6 ng/mL、24.3 ng/mL和21.7 ng/mL。拟除虫菊酯结构类似物的交叉反应率均低于0.6%。六种黑茶被用于检测氰戊菊酯单克隆抗体的实际应用。含 30% 甲醇的 PBS 中抗氰戊菊酯单克隆抗体的灵敏度 IC50 为 29.12 ng/mL。并初步研制出LOD为10.0 ng/mL、LDR为18.9-357 ng/mL的乳胶微球免疫层析试纸条。成功制备了特异性灵敏的氰戊菊酯单克隆抗体，并应用于黑茶（普洱茶、六堡茶、茯砖茶、青砖茶、恩施黑茶和富硒恩施黑茶）中氰戊菊酯的检测。