Epiretinal Amniotic Membrane in Complicated Retinal Detachment: a Clinical and In Vitro Safety Assessment,Ophthalmology and Therapy

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Epiretinal Amniotic Membrane in Complicated Retinal Detachment: a Clinical and In Vitro Safety Assessment
Ophthalmology and Therapy ( IF 2.6 ) Pub Date : 2023-03-11 , DOI: 10.1007/s40123-023-00695-z
Anna Hillenmayer _{1,

2} , Christian M Wertheimer _{1,

2} , Maximilian-Joachim Gerhard ₁ , Siegfried G Priglinger ₁ , Andreas Ohlmann ₁ , Armin Wolf _{1,

2}

Affiliation

Introduction

Amniotic membrane (AM) is a popular treatment for external ocular diseases. First intraocular implantations in other diseases reported promising results. Here, we review three cases of intravitreal epiretinal human AM (iehAM) transplantation as an adjunct treatment for complicated retinal detachment and analyze clinical safety. Possible cellular rejection reactions against the explanted iehAM were evaluated and its influence was assessed on three retinal cell lines in vitro.

Methods

Three patients with complicated retinal detachment and implanted iehAM during pars plana vitrectomy are retrospectively presented. After removal of the iehAM at subsequent surgery, tissue-specific cellular responses were studied by light microscopy and immunohistochemical staining. We investigated the influence of AM in vitro on retinal pigment epithelial cells (ARPE-19), Müller cells (Mio-M1), and differentiated retinal neuroblasts (661W) . An anti-histone DNA ELISA for cell apoptosis, a BrdU ELISA for cell proliferation, a WST-1 assay for cell viability, and a live/dead assay for cell death were performed.

Results

Despite the severity of the retinal detachment, stable clinical outcomes were obtained in all three cases. Immunostaining of the explanted iehAM showed no evidence of cellular immunological rejection. In vitro, there was no statistical significant change in cell death or cell viability nor were proliferative effects detected on ARPE-19, Müller cells, and retinal neuroblasts exposed to AM.

Conclusion

iehAM was a viable adjuvant with many potential benefits for treatment of complicated retinal detachment. Our investigations could not detect any signs of rejection reactions or toxicity. Further studies are needed to evaluate this potential in more detail.

中文翻译：

复杂性视网膜脱离中的视网膜前羊膜：临床和体外安全性评估

介绍

羊膜 (AM) 是一种流行的外眼疾病治疗方法。其他疾病的首次眼内植入报告了有希望的结果。在这里，我们回顾了三例玻璃体内视网膜前人 AM (iehAM) 移植作为复杂性视网膜脱离的辅助治疗，并分析了临床安全性。评估了针对外植 iehAM 的可能细胞排斥反应，并在体外评估了其对三种视网膜细胞系的影响。

方法

回顾性介绍了在睫状体玻璃体切除术中植入 iehAM 的三例复杂性视网膜脱离患者。在随后的手术中去除 iehAM 后，通过光学显微镜和免疫组织化学染色研究了组织特异性细胞反应。我们研究了 AM 在体外对视网膜色素上皮细胞 (ARPE-19)、Müller 细胞 (Mio-M1) 和分化的视网膜成神经细胞 (661W) 的影响。进行了用于细胞凋亡的抗组蛋白 DNA ELISA、用于细胞增殖的 BrdU ELISA、用于细胞活力的 WST-1 测定和用于细胞死亡的活/死测定。