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Digested extracellular DNA shortens the anodic startup of microbial electrolysis cell
Science of the Total Environment ( IF 8.2 ) Pub Date : 2023-03-07 , DOI: 10.1016/j.scitotenv.2023.162642
Xiangyu Han 1 , Jiaman Wang 2 , Jingjing Zhang 1 , Baohong Han 1 , Ning Mei 1 , Runchuan Fan 3 , Jing Zhao 1 , Hong Yao 1 , Xiaohua Yu 1 , Weiwei Cai 1
Affiliation  

While the multiple functions of extracellular DNA (exDNA) in biofilm formation and electron transfer have been extensively studied in pure culture, its role in mixed anodic biofilm was still unknown. In this study, we employed DNase I enzyme to digest exDNA, thereby investigating its role in anodic biofilm formation based on the performance of four microbial electrolysis cells (MECs) groups with different DNase I enzyme concentration (0, 0.05, 0.1, 0.5 mg/mL). The responding time to reach 60 % maximum current of treatment group with DNase I enzyme has been significantly reduced to 83 %–86 % of the blank group (t-test, p < 0.01), indicating the exDNA digestion could promote the biofilm formation at the early stage. The anodic coulombic efficiency was enhanced by 10.74– 54.42 % in treatment group (t-test, p < 0.05), which could be ascribed to the higher absolute abundance of exoelectrogens. The lower relative abundance of exoelectrogens indicated the DNase I enzyme addition was beneficial for the enrichment of extensive species rather than exoelectrogens. As the DNase I enzyme augments the fluorescence signal of exDNA distribution in the small molecular weight region, implying the short chain exDNA could contribute to the biomass enhancement via boosting the most species enrichment. Furthermore, the exDNA alteration improved the complexity of microbial network. Our findings provide a new insight into the role of exDNA in the extracellular matrix of anodic biofilms.

中文翻译:


消化的细胞外 DNA 缩短了微生物电解槽的阳极启动



虽然细胞外 DNA (exDNA) 在生物膜形成和电子转移中的多种功能已在纯培养物中得到广泛研究,但其在混合阳极生物膜中的作用仍然未知。在这项研究中,我们采用 DNase I 酶消化 exDNA,从而根据具有不同 DNase I 酶浓度 (0、0.05、0.1、0.5 mg/mL) 的四个微生物电解细胞 (MEC) 组的性能研究其在阳极生物膜形成中的作用。DNase I 酶处理组达到 60 % 最大电流的响应时间已显着缩短至空白组的 83 %–86 %(t 检验,p < 0.01),表明 exDNA 消化可以促进早期生物膜的形成。处理组阳极库仑效率提高了 10.74-54.42% (t 检验,p < 0.05),这可能归因于较高的外电原绝对丰度。外电原的较低相对丰度表明 DNase I 酶的添加有利于广泛物种的富集,而不是外电原。由于 DNase I 酶增强了 exDNA 在小分子量区域分布的荧光信号,这意味着短链 exDNA 可以通过促进大多数物种富集来促进生物量增强。此外,exDNA 改变提高了微生物网络的复杂性。我们的研究结果为 exDNA 在阳极生物膜细胞外基质中的作用提供了新的见解。
更新日期:2023-03-07
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