In the present study, gelatin (Ge) and polyvinyl alcohol (PVA) were used in order to synergize and strengthen as much as possible the various properties of the final matrix. To create antimicrobial properties in wound dressings, quaternary ammonium salt with silane was synthesized based on natural castor oil (CO), with the ability to bind to the polymer-siloxane network structure. The characterization and confirmation of the final structure and the detailed investigation of the chemical synthesis path of the antimicrobial agent were performed through the analysis of the data obtained from the ATR- FTIR methods. The high gel content values of the prepared films indicate the favorable cross-linking of the polymers in the structure of the wound dressings. Also, the observation of water absorption of wound dressings in the range of 220.1–351.6% confirms the high ability of polymer wound dressings to retain and absorb wound secretions. The level of hydrophilicity of the wound dressing surface was investigated by measuring the contact angle. The contact angles of all the films were less than 90º, which indicates the hydrophilic nature of the prepared films. The water vapor transmission rate (WVTR) of the wound dressings prepared in the range of 2224–2243 g/ m²/ day showed that the samples have the necessary ability as absorbent wound dressings to create a moist environment in the bed of wounds with medium to heavy discharge. The results obtained from the tensile test in both dry and wet conditions showed that the tensile strength of the prepared films was in a suitable range and comparable to the tensile strength of the skin. This combination of antibiotic/inhibitor with appropriate charge density and hydrophobicity is able to interact favorably with the bacterial membrane and has sufficient antibiotic properties against Gram-positive and Gram-negative bacteria.