Characterization of 25 new microsatellite markers for the green turtle (Chelonia mydas) and cross-species amplification in other marine turtle species,Molecular Biology Reports

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Characterization of 25 new microsatellite markers for the green turtle (Chelonia mydas) and cross-species amplification in other marine turtle species
Molecular Biology Reports ( IF 2.6 ) Pub Date : 2023-03-06 , DOI: 10.1007/s11033-023-08341-4
Violaine Dolfo ₁ , Emilie Boissin _{1,

2} , Miri Tatarata ₃ , Serge Planes _{1,

2}

Affiliation

Background

The green sea turtle, Chelonia mydas, is a migratory species with a strong natal homing behavior leading to a complex population structure worldwide. The species has suffered severe declines in local populations; it is therefore crucial to understand its population dynamics and genetic structure to adopt appropriate management policies. Here, we describe the development of 25 new microsatellite markers specific to C. mydas and suitable for these analyses.

Methods and results

They were tested on 107 specimens from French Polynesia. An average allelic diversity of 8 alleles per locus was reported and observed heterozygosity ranged from 0.187 to 0.860. Ten loci were significantly deviant from the Hardy-Weinberg equilibrium, and 16 loci showed a moderate to high level of linkage disequilibrium (4–22%). The overall F_is was positive (0.034, p-value < 0.001), and sibship analysis revealed 12 half- or full-sibling dyads, suggesting possible inbreeding in this population. Cross-amplification tests were performed on two other marine turtle species, Caretta caretta and Eretmochelys imbricata. All loci successfully amplified on these two species, though 1 to 5 loci were monomorphic.

Conclusion

These new markers will not only be relevant for further analyses on the population structure of the green turtle and the two other species, but they will also be invaluable for parentage studies, for which a high number of polymorphic loci are necessary. This can provide important insight into male reproductive behavior and migration, an aspect of sea turtle biology that is of critical importance for the conservation of the species.

中文翻译：

绿海龟 (Chelonia mydas) 的 25 个新微卫星标记的特征和其他海龟物种的跨物种扩增

背景

绿海龟Chelonia mydas是一种迁徙物种，具有强烈的出生归巢行为，导致世界范围内的种群结构复杂。该物种的当地种群数量严重下降；因此，了解其种群动态和遗传结构以采取适当的管理政策至关重要。在这里，我们描述了 25 个特定于C. mydas并适用于这些分析的新微卫星标记的开发。

方法和结果

他们对来自法属波利尼西亚的 107 个样本进行了测试。报告了每个基因座 8 个等位基因的平均等位基因多样性，观察到的杂合性范围为 0.187 至 0.860。10 个基因座显着偏离 Hardy-Weinberg 平衡，16 个基因座显示中度至高度连锁不平衡 (4–22%)。总体 F_为正（0.034，p 值 < 0.001），同胞分析显示有 12 个同胞或同胞对，表明该人群可能存在近亲繁殖。对另外两种海龟Caretta caretta和Eretmochelys imbricata进行了交叉扩增测试。尽管有 1 到 5 个基因座是单态的，但所有基因座都在这两个物种上成功扩增。