Molecular Biology Reports ( IF 2.6 ) Pub Date : 2023-03-04 , DOI: 10.1007/s11033-023-08328-1 Priya Pardeshi 1 , Pravin Jadhav 1 , Sanjay Sakhare 1 , Rajkumar Zunjare 2 , Darasing Rathod 1 , Priti Sonkamble 1 , Ranjit Saroj 2 , Philips Varghese 3
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Background
Vegetable soybean seeds are among the most popular and nutrient-dense beans in the world due to their delicious flavor, high yield, superior nutritional value, and low trypsin content. There is significant potential for this crop that Indian farmers do not fully appreciate because of the limited germplasm range. Therefore, the current study aims to identify the diverse lines of vegetable soybean and explore the diversity produced by hybridizing grain and vegetable-type soybean varieties. Indian researchers have not yet published work describing and analysing novel vegetable soybean for microsatellite markers and morphological traits.
Methods and results
Sixty polymorphic SSR markers and 19 morphological traits were used to evaluate the genetic diversity of 21 newly developed vegetable soybean genotypes. A total of 238 alleles, ranging from 2 to 8, were found, with a mean of 3.97 alleles per locus. The polymorphism information content varied from 0.05 to 0.85, with an average of 0.60. A variation of 0.25–0.58 with a mean of 0.43 was observed for Jaccard’s dissimilarity coefficient.
Conclusion
The diverse genotypes identified can be helpful to understand the genetics of vegetable soybean traits and can be used in improvement programs; study also explains the utility of SSR markers for diversity analysis of vegetable soybean. Here, we identified the highly informative SSRs with PIC > 0.80 (satt199, satt165, satt167, satt191, satt183, satt202, and satt126), which apply to genetic structure analysis, mapping strategies, polymorphic marker surveys, and background selection in genomics-assisted breeding.
中文翻译：
新型菜用大豆 [Glycine max (L.) Merrill] 的形态学和微卫星标记表征及多样性分析
背景
菜用大豆种子因其味道鲜美、产量高、营养价值优越、胰蛋白酶含量低而成为世界上最受欢迎、营养丰富的豆类之一。由于种质范围有限，印度农民尚未充分认识到这种作物的巨大潜力。因此，本研究旨在鉴定菜用大豆的多样性系，并探索粮用型大豆品种与菜用型大豆品种杂交产生的多样性。印度研究人员尚未发表描述和分析新型蔬菜大豆的微卫星标记和形态特征的论文。
方法和结果
利用60个多态性SSR标记和19个形态性状评价了21个新开发的菜用大豆基因型的遗传多样性。共发现 238 个等位基因，范围为 2 至 8 个，平均每个位点有 3.97 个等位基因。多态性信息含量为0.05～0.85，平均为0.60。 Jaccard 相异系数的变化范围为 0.25–0.58，平均值为 0.43。
结论
鉴定出的不同基因型有助于了解菜用大豆性状的遗传学，并可用于改良计划；研究还解释了 SSR 标记在菜用大豆多样性分析中的效用。在这里，我们鉴定了 PIC > 0.80 的信息丰富的 SSR（ satt199、satt165、satt167、satt191、satt183、satt202和satt126 ），它们适用于基因组学辅助中的遗传结构分析、作图策略、多态性标记调查和背景选择配种。
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