PIP2 Alteration Caused by Elastic Modulus and Tropism of Electrospun Scaffolds Facilitates Altered BMSCs Proliferation and Differentiation,Advanced Materials

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PIP2 Alteration Caused by Elastic Modulus and Tropism of Electrospun Scaffolds Facilitates Altered BMSCs Proliferation and Differentiation
Advanced Materials ( IF 27.4 ) Pub Date : 2023-03-03 , DOI: 10.1002/adma.202212272
Chen Gao ₁ , Yulin Lai _{2,

3} , Liang Cheng _{2,

3} , Yifan Cheng _{2,

3} , Anqi Miao _{2,

3} , Jialong Chen ₃ , Runhuai Yang _{2,

3} , Fei Xiong ₁

Affiliation

Aligned submicron fibers have played an essential role in inducing stem cell proliferation and differentiation. In this study, it is aimed to identify the differential causes of stem cell proliferation and differentiation between bone marrow mesenchymal stem cells (BMSCs) on aligned-random fibers with different elastic modulus, and to change the differential levels through a regulatory mechanism mediated by B-cell lymphoma 6 protein(BCL-6) and miRNA-126-5p(miR-126-5p). The results showed that phosphatidylinositol(4,5)bisphosphate alterations are found in the aligned fibers compared with the random fibers, which has a regular and oriented structure, excellent cytocompatibility, regular cytoskeleton, and high differentiation potential. The same trend is actual for the aligned fibers with a lower elastic modulus. The level of proliferative differentiation genes in cells is altered by BCL-6 and miR-126-5p mediated regulatory mechanisms to make the cell distribution nearly consistent with the cell state on low elastic modulus aligned fibers. This work demonstrates the reason for the difference of cells between the two kinds of fibers and on fibers with different elastic modulus. These findings provide more insights for understanding the gene-level regulation of cell growth in tissue engineering.

中文翻译：

静电纺支架的弹性模量和趋向性引起的 PIP2 改变促进了改变的 BMSCs 增殖和分化

对齐的亚微米纤维在诱导干细胞增殖和分化中发挥了重要作用。本研究旨在确定不同弹性模量排列随机纤维上骨髓间充质干细胞（BMSCs）干细胞增殖和分化的差异原因，并通过B介导的调控机制改变差异水平。 -细胞淋巴瘤6蛋白（BCL-6）和miRNA-126-5p（miR-126-5p）。结果表明，与随机纤维相比，排列纤维中存在磷脂酰肌醇（4,5）二磷酸盐改变，具有规则和定向的结构，良好的细胞相容性，规则的细胞骨架和高分化潜能。对于具有较低弹性模量的对齐纤维，实际情况也是如此。BCL-6 和 miR-126-5p 介导的调节机制改变细胞中增殖分化基因的水平，使细胞分布与低弹性模量对齐纤维上的细胞状态几乎一致。这项工作证明了两种纤维和具有不同弹性模量的纤维之间的细胞差异的原因。这些发现为理解组织工程中细胞生长的基因水平调控提供了更多见解。

更新日期：2023-03-03

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