Electrochemically Etched Tapered-Tip Stainless-Steel Electrospray-Ionization Emitters for Capillary Electrophoresis–Mass Spectrometry,Journal of Proteome Research

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Electrochemically Etched Tapered-Tip Stainless-Steel Electrospray-Ionization Emitters for Capillary Electrophoresis–Mass Spectrometry
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2023-03-03 , DOI: 10.1021/acs.jproteome.3c00076
Jordan T Aerts ₁ , Per E Andrén _{1,

2} , Erik T Jansson ₁

Affiliation

We have used household consumables to facilitate electrochemical etching of stainless-steel hypodermic tubing to produce tapered-tip emitters suitable for electrospray ionization for use in mass spectrometry. The process involves the use of 1% oxalic acid and a 5 W USB power adapter, commonly known as a phone charger. Further, our method avoids the otherwise commonly used strong acids that entail chemical hazards: concentrated HNO₃ for etching stainless steel, or concentrated HF for etching fused silica. Hence, we here provide a convenient and self-inhibiting procedure with minimal chemical hazards to manufacture tapered-tip stainless-steel emitters. We show its performance in metabolomic analysis with CE–MS of a tissue homogenate where the metabolites acetylcarnitine, arginine, carnitine, creatine, homocarnosine, and valerylcarnitine were identified, all with basepeak separated electropherograms, within <6 min of separation. The mass spectrometry data are freely available through the MetaboLight public data repository via access number MTBLS7230.

中文翻译：

用于毛细管电泳-质谱分析的电化学蚀刻锥形尖端不锈钢电喷雾电离发射器

我们使用家用耗材促进不锈钢皮下注射管的电化学蚀刻，以生产适用于电喷雾电离的锥形尖端发射器，用于质谱分析。该过程涉及使用 1% 的草酸和 5 W USB 电源适配器，通常称为手机充电器。此外，我们的方法避免了通常使用的会带来化学危害的强酸：浓 HNO ₃用于蚀刻不锈钢，或浓缩 HF 用于蚀刻熔融石英。因此，我们在这里提供了一种方便且自我抑制的程序，以最小的化学危害来制造锥形尖端不锈钢发射器。我们展示了其在组织匀浆的 CE-MS 代谢组学分析中的性能，其中代谢物乙酰肉碱、精氨酸、肉碱、肌酸、高肌肽和戊酰肉碱在分离后 <6 分钟内得到了基峰分离电泳图谱的鉴定。质谱数据可通过访问号 MTBLS7230 通过 MetaboLight 公共数据存储库免费获得。

更新日期：2023-03-03

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