Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2023-03-03 , DOI: 10.1007/s00216-023-04606-w
Rui Wang 1 , Hui-Da Li 1 , Ying Cao 1 , Zi-Yi Wang 1 , Ting Yang 1 , Jian-Hua Wang 1
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Viruses are changing the biosensing and biomedicine landscape due to their multivalency, orthogonal reactivities, and responsiveness to genetic modifications. As the most extensively studied phage model for constructing a phage display library, M13 phage has received much research attention as building blocks or viral scaffolds for various applications including isolation/separation, sensing/probing, and in vivo imaging. Through genetic engineering and chemical modification, M13 phages can be functionalized into a multifunctional analysis platform with various functional regions conducting their functionality without mutual disturbance. Its unique filamentous morphology and flexibility also promoted the analytical performance in terms of target affinity and signal amplification. In this review, we mainly focused on the application of M13 phage in the analytical field and the benefit it brings. We also introduced several genetic engineering and chemical modification approaches for endowing M13 with various functionalities, and summarized some representative applications using M13 phages to construct isolation sorbents, biosensors, cell imaging probes, and immunoassays. Finally, current issues and challenges remaining in this field were discussed and future perspectives were also proposed.
Graphical Abstract
中文翻译：
M13 噬菌体：用于高度特异性分析平台的多功能构建模块
病毒由于其多价性、正交反应性和对基因修饰的反应性，正在改变生物传感和生物医学领域。作为构建噬菌体展示文库研究最广泛的噬菌体模型，M13 噬菌体作为构建块或病毒支架，用于各种应用（包括分离/分离、传感/探测和体内成像），受到了广泛的研究关注。通过基因工程和化学修饰，M13噬菌体可以功能化为多功能分析平台，各个功能区互不干扰地发挥其功能。其独特的丝状形态和灵活性也提升了目标亲和力和信号放大方面的分析性能。在这篇综述中，我们主要关注M13噬菌体在分析领域的应用及其带来的好处。我们还介绍了几种赋予 M13 各种功能的基因工程和化学修饰方法，并总结了使用 M13 噬菌体构建分离吸附剂、生物传感器、细胞成像探针和免疫分析的一些代表性应用。最后，讨论了该领域当前存在的问题和挑战，并提出了未来的前景。
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