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Pocket-Size Wireless Nanoelectrospray Ionization Mass Spectrometry for Metabolic Analysis of Salty Biofluids and Single Cells
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-02 , DOI: 10.1021/acs.analchem.2c04268 Yuze Li 1, 2 , Ke Jia 2, 3 , Yixin Pan 2, 3 , Jing Han 2 , Junyu Chen 2, 3 , Yiran Wang 2, 3 , Xiaobing Ma 4 , Hongwei Chen 5 , Shengjie Wang 5 , Dongcheng Xie 5 , Caiqiao Xiong 2 , Zongxiu Nie 1, 2, 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-03-02 , DOI: 10.1021/acs.analchem.2c04268 Yuze Li 1, 2 , Ke Jia 2, 3 , Yixin Pan 2, 3 , Jing Han 2 , Junyu Chen 2, 3 , Yiran Wang 2, 3 , Xiaobing Ma 4 , Hongwei Chen 5 , Shengjie Wang 5 , Dongcheng Xie 5 , Caiqiao Xiong 2 , Zongxiu Nie 1, 2, 3
Affiliation
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Analysis of volume-limited biological samples such as single cells and biofluids not only benefits clinical purposes but also promotes fundamental research in life sciences. Detection of these samples, however, imposes strict requirements on measurement performance because of the minimal volume and concentrated salts of the samples. Herein, we developed a self-cleaning nanoelectrospray ionization device powered by a pocket-size “MasSpec Pointer” (MSP-nanoESI) for metabolic analysis of salty biological samples with limited volume. The self-cleaning effect induced by Maxwell–Wagner electric stress helps with keeping the borosilicate glass capillary tip free from clogging and thus increasing salt tolerance. This device possesses a high sample economy (about 0.1 μL per test) due to its pulsed high voltage supply, sampling method (dipping the nanoESI tip into analyte solution), and contact-free electrospray ionization (ESI) (the electrode does not touch the analyte solution during ESI). High repeatable results could be acquired by the device with a relative standard deviation (RSD) of 1.02% for voltage output and 12.94% for MS signals of caffeine standard. Single MCF-7 cells were metabolically analyzed directly from phosphate buffered saline, and two types of untreated cerebrospinal fluid from hydrocephalus patients were distinguished with 84% accuracy. MSP-nanoESI gets rid of the bulky apparatus and could be held in hand or put into one’s pocket for transportation, and it could operate for more than 4 h without recharge. We believe this device will boost scientific research and clinical usage of volume-limited biological samples with high-concentration salts in a low-cost, convenient, and rapid manner.
中文翻译：
用于含盐生物流体和单细胞代谢分析的袖珍型无线纳米电喷雾电离质谱仪
分析体积有限的生物样品，如单细胞和生物流体，不仅有利于临床目的，而且还能促进生命科学的基础研究。然而，由于样品体积小且盐分浓度高，检测这些样品对测量性能提出了严格的要求。在此，我们开发了一种由袖珍型“MasSpec 指针”(MSP-nanoESI) 提供动力的自清洁纳米电喷雾电离装置，用于有限体积的含盐生物样品的代谢分析。Maxwell-Wagner 电应力引起的自清洁效应有助于防止硼硅酸盐玻璃毛细管尖端堵塞，从而提高耐盐性。由于其脉冲高压电源，该设备具有很高的样品经济性（每次测试约 0.1 μL），采样方法（将 nanoESI 尖端浸入分析物溶液）和无接触电喷雾电离 (ESI)（电极在 ESI 期间不接触分析物溶液）。该设备可以获得高重复性结果，电压输出的相对标准偏差 (RSD) 为 1.02%，咖啡因标准品的 MS 信号为 12.94%。直接从磷酸盐缓冲盐水中对单个 MCF-7 细胞进行代谢分析，区分两种类型的未经处理的脑积水患者脑脊液，准确率为 84%。MSP-nanoESI摆脱了笨重的仪器，可以拿在手里或放在口袋里携带，无需充电即可运行4小时以上。
更新日期：2023-03-02
中文翻译：
用于含盐生物流体和单细胞代谢分析的袖珍型无线纳米电喷雾电离质谱仪
分析体积有限的生物样品，如单细胞和生物流体，不仅有利于临床目的，而且还能促进生命科学的基础研究。然而，由于样品体积小且盐分浓度高，检测这些样品对测量性能提出了严格的要求。在此，我们开发了一种由袖珍型“MasSpec 指针”(MSP-nanoESI) 提供动力的自清洁纳米电喷雾电离装置，用于有限体积的含盐生物样品的代谢分析。Maxwell-Wagner 电应力引起的自清洁效应有助于防止硼硅酸盐玻璃毛细管尖端堵塞，从而提高耐盐性。由于其脉冲高压电源，该设备具有很高的样品经济性（每次测试约 0.1 μL），采样方法（将 nanoESI 尖端浸入分析物溶液）和无接触电喷雾电离 (ESI)（电极在 ESI 期间不接触分析物溶液）。该设备可以获得高重复性结果，电压输出的相对标准偏差 (RSD) 为 1.02%，咖啡因标准品的 MS 信号为 12.94%。直接从磷酸盐缓冲盐水中对单个 MCF-7 细胞进行代谢分析，区分两种类型的未经处理的脑积水患者脑脊液，准确率为 84%。MSP-nanoESI摆脱了笨重的仪器，可以拿在手里或放在口袋里携带，无需充电即可运行4小时以上。
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