Age-induced erectile dysfunction (ED) is a convoluted medical condition, and restoring erectile function (EF) under geriatric conditions is highly complicated. Platelet-rich plasma (PRP) treatment is an inexpensive cell-based therapeutic strategy. We have aimed to restore EF in aged-ED rats with PRP as a therapeutic tool. Male rats were grouped into aged and young according to age. The young rats were considered as normal control (NC) and treated with saline. Aged were further divided into 2 groups and treated with intracavernous (IC) PRP and saline. Treatment was scheduled at the 9th and 10th week for NC and 41th and 42th week for aged-ED rats, with EF analysis scheduled on the 12th week for NC and 44th week for aged-ED rats, respectively. Erectile response, immunofluorescence staining, and electron microscopic analyses were performed. IC PRP treatment effectively reduced prostate hyperplasia (PH). EF response indicated a significant increase in crucial EF parameters in PRP-treated aged-ED rats. Histological evidence denoted a rigid and restored development of tunica adventitia of the dorsal artery, decreased vacuolation of the dorsal penile nerve, and structural expansion of the epineurium. Masson's trichrome and immunostaining results affirmed an elevated expression of α-smooth muscle actin (α-SMA) in the corpus cavernosum (CC). Ultrastructure findings revealed that PRP effectively rejuvenated degenerating nerves, preserved endothelium and adherent junctions of corporal smooth muscle, and restored the axonal scaffolds by upregulating neurofilament-H (NF-H) expression. Finally, PRP enhanced neural stability by enhancing the axonal remyelination processes in aged-ED rats. Hence, PRP treatment was proven to restore EF in aged-ED rats, which was considered a safe, novel, cost-effective, and hassle-free strategy for EF restoration in geriatric patients.

中文翻译：

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海绵体内注射富含血小板的血浆通过减少衰老诱导的勃起功能障碍大鼠模型的前列腺增生证据来逆转慢性海绵体神经变性的勃起功能障碍

年龄诱发的勃起功能障碍 (ED) 是一种复杂的医学病症，在老年病症下恢复勃起功能 (EF) 非常复杂。富血小板血浆 (PRP) 治疗是一种廉价的基于细胞的治疗策略。我们的目标是使用 PRP 作为治疗工具恢复老年 ED 大鼠的 EF。雄性大鼠按年龄分为老年组和幼年组。年轻大鼠被视为正常对照 (NC) 并用盐水处理。老年人被进一步分为 2 组，并用海绵体内 (IC) PRP 和盐水进行治疗。NC 的治疗安排在第 9 周和第 10 周，老年 ED 大鼠的治疗安排在第 41 周和 42 周，EF 分析分别安排在 NC 的第 12 周和老年 ED 大鼠的第 44 周。进行了勃起反应、免疫荧光染色和电子显微镜分析。IC PRP 治疗有效地减少了前列腺增生 (PH)。EF 反应表明 PRP 处理的老年 ED 大鼠的关键 EF 参数显着增加。组织学证据表明背动脉外膜坚硬且恢复发育，阴茎背神经空泡减少，神经外膜结构扩张。Masson 的三色和免疫染色结果证实了海绵体 (CC) 中 α-平滑肌肌动蛋白 (α-SMA) 的表达升高。超微结构研究结果表明，PRP 有效地使退化的神经恢复活力，保护内皮细胞和下体平滑肌的粘附连接，并通过上调神经丝-H (NF-H) 表达来恢复轴突支架。最后，PRP 通过增强老年 ED 大鼠的轴突髓鞘再生过程来增强神经稳定性。