未观察到不良反应水平的脱氧雪腐镰刀菌烯醇通过 JAK2/STAT3 信号通路在小鼠中加重 DSS 诱导的结肠炎,Journal of Agricultural and Food Chemistry

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未观察到不良反应水平的脱氧雪腐镰刀菌烯醇通过 JAK2/STAT3 信号通路在小鼠中加重 DSS 诱导的结肠炎
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-02-27 , DOI: 10.1021/acs.jafc.3c00252
Fang Gan _{1,

2,

3} , Ziman Lin _{1,

2,

3} , Jiangyu Tang _{1,

2,

3} , Xingxiang Chen _{1,

2,

3} , Kehe Huang _{1,

2,

3}

Affiliation

炎症性肠病 (IBD) 的病因涉及复杂的遗传和环境因素，例如霉菌毒素污染。脱氧雪腐镰刀菌烯醇 (DON) 是一种众所周知的霉菌毒素，会污染食物和饲料，并可引起肠道损伤和炎症反应。很多食物中DON的剂量也低于限值，虽然DON的剂量超过了限值。本研究旨在评估无毒剂量的 DON 对葡聚糖硫酸钠 (DSS) 诱导的小鼠结肠炎的影响及其机制。结果显示每天 50 μg/kg bw 的无毒剂量的 DON 加剧了 DSS 诱导的小鼠结肠炎，表现为疾病活动指数增加、结肠长度缩短、形态学损伤增加、occludin 和粘蛋白 2 表达减少、IL-1β 增加和 TNF-α 的表达，并降低 IL-10 的表达。每天 50 μg/kg bw 的 DON 增强了 DSS 诱导的 JAK2/STAT3 磷酸化。添加 JAK2 抑制剂 AG490 通过逆转形态学损伤减弱 DON 对 DSS 诱导的结肠炎的加重作用，occludin 和粘蛋白 2 表达增加，IL-1β 和 TNF-α 表达增加，IL-10 表达减少。总之，无毒剂量的呕吐毒素可通过 JAK2/STAT3 信号通路加重 DSS 诱导的结肠炎。这表明低于标准限量剂量的 DON 也存在 IBD 风险，并可能对人和动物的健康造成危害，这可以为确定 DON 限量提供依据。occludin和粘蛋白2表达增加，IL-1β和TNF-α表达增加，IL-10表达减少。总之，无毒剂量的呕吐毒素可通过 JAK2/STAT3 信号通路加重 DSS 诱导的结肠炎。这表明低于标准限量剂量的 DON 也存在 IBD 风险，并可能对人和动物的健康造成危害，这可以为确定 DON 限量提供依据。occludin和粘蛋白2表达增加，IL-1β和TNF-α表达增加，IL-10表达减少。总之，无毒剂量的呕吐毒素可通过 JAK2/STAT3 信号通路加重 DSS 诱导的结肠炎。这表明低于标准限量剂量的 DON 也存在 IBD 风险，并可能对人和动物的健康造成危害，这可以为确定 DON 限量提供依据。

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Deoxynivalenol at No-Observed Adverse-Effect Levels Aggravates DSS-Induced Colitis through the JAK2/STAT3 Signaling Pathway in Mice

The etiology of inflammatory bowel diseases (IBDs) involves complex genetic and environmental factors such as mycotoxin contamination. Deoxynivalenol (DON), a well-known mycotoxin, contaminates food and feed and can induce intestinal injury and inflammatory response. The dose of DON in many foods is also below the limit, although the dose of DON exceeds the limit. The present study aims to evaluate the effects of the nontoxic dose of DON on colitis induced by dextran sodium sulfate (DSS) and the mechanism in mice. The results showed a nontoxic dose of DON at 50 μg/kg bw per day exacerbated DSS-induced colitis in mice as demonstrated by increased disease activity index, decreased colon length, increased morphological damage, decreased occludin and mucoprotein 2 expression, increased IL-1β and TNF-α expression, and decreased IL-10 expression. DON at 50 μg/kg bw per day enhanced JAK2/STAT3 phosphorylation induced by DSS. Adding JAK2 inhibitor AG490 attenuated the aggravating effects of DON on DSS-induced colitis by reversing the morphological damage, occludin and mucoprotein 2 expression increased, IL-1β and TNF-α expression increased, and IL-10 expression decreased. Taken together, a nontoxic dose of DON could aggravate DSS-induced colitis via the JAK2/STAT3 signaling pathway. This suggests that DON, below the standard limit dose, is also a risk for IBD and may be harmful to the health of humans and animals, which could provide the basis for establishing limits for DON.

更新日期：2023-02-27

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