细胞分裂素拮抗剂在根肿病进展中的作用。,Biomolecules

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细胞分裂素拮抗剂在根肿病进展中的作用。
Biomolecules ( IF 4.8 ) Pub Date : 2023-02-05 , DOI: 10.3390/biom13020299
Jana Bíbová ₁ , Veronika Kábrtová ₁ , Veronika Večeřová ₁ , Zuzana Kučerová ₂ , Martin Hudeček ₁ , Lenka Plačková ₁ , Ondřej Novák ₁ , Miroslav Strnad ₁ , Ondřej Plíhal ₁

Affiliation

十字花科植物 Plasmodiophora brassicae 是一种专性生物营养型病原体，可引起十字花科植物根肿病。受感染的植物器官发生深刻的形态变化，根部形成特征性的虫瘿，叶子褪绿和脱落。虫瘿的形成过程受根肿病原体整个生命周期中内源性植物激素水平及时变化的控制。两种植物激素细胞分裂素和生长素的稳态似乎对俱乐部发展至关重要。为了研究细胞分裂素和生长素在虫瘿形成中的作用，我们使用了感染根肿病的拟南芥的代谢组学和转录组学分析，重点关注症状更为明显的疾病晚期。三种细胞分裂素受体 AHK2、AHK3 和 CRE1/AHK4 的功能缺失突变体，被用来进一步研究细胞分裂素对疾病进展的稳态反应；ahk 双突变体出现了该疾病的特征性症状，尽管强度不同。最易患根肿病的是 ahk3 ahk4 双突变体，正如通过测量其光合性能所揭示的那样。植物激素水平的量化和细胞分裂素拮抗剂 PI-55 的药物治疗显示内源性细胞分裂素和生长素水平发生显着变化，这表现为不同基因型疾病症状发展的增强和减少。最易患根肿病的是 ahk3 ahk4 双突变体，正如通过测量其光合性能所揭示的那样。植物激素水平的量化和细胞分裂素拮抗剂 PI-55 的药物治疗显示内源性细胞分裂素和生长素水平发生显着变化，这表现为不同基因型疾病症状发展的增强和减少。最易患根肿病的是 ahk3 ahk4 双突变体，正如通过测量其光合性能所揭示的那样。植物激素水平的量化和细胞分裂素拮抗剂 PI-55 的药物治疗显示内源性细胞分裂素和生长素水平发生显着变化，这表现为不同基因型疾病症状发展的增强和减少。

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The Role of a Cytokinin Antagonist in the Progression of Clubroot Disease.

Plasmodiophora brassicae is an obligate biotrophic pathogen causing clubroot disease in cruciferous plants. Infected plant organs are subject to profound morphological changes, the roots form characteristic galls, and the leaves are chlorotic and abscise. The process of gall formation is governed by timely changes in the levels of endogenous plant hormones that occur throughout the entire life cycle of the clubroot pathogen. The homeostasis of two plant hormones, cytokinin and auxin, appears to be crucial for club development. To investigate the role of cytokinin and auxin in gall formation, we used metabolomic and transcriptomic profiling of Arabidopsis thaliana infected with clubroot, focusing on the late stages of the disease, where symptoms were more pronounced. Loss-of-function mutants of three cytokinin receptors, AHK2, AHK3, and CRE1/AHK4, were employed to further study the homeostasis of cytokinin in response to disease progression; ahk double mutants developed characteristic symptoms of the disease, albeit with varying intensity. The most susceptible to clubroot disease was the ahk3 ahk4 double mutant, as revealed by measuring its photosynthetic performance. Quantification of phytohormone levels and pharmacological treatment with the cytokinin antagonist PI-55 showed significant changes in the levels of endogenous cytokinin and auxin, which was manifested by both enhanced and reduced development of disease symptoms in different genotypes.

更新日期：2023-02-05

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