Kitul，一种具有抗糖尿病样作用的食用植物：减少葡萄糖刺激的 RIN-5F 胰腺 β 细胞中的细胞内活性物质并减少活化的 RAW 264.7 巨噬细胞中的促炎介质,Food Research International

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Kitul，一种具有抗糖尿病样作用的食用植物：减少葡萄糖刺激的 RIN-5F 胰腺 β 细胞中的细胞内活性物质并减少活化的 RAW 264.7 巨噬细胞中的促炎介质
Food Research International ( IF 7.0 ) Pub Date : 2023-02-24 , DOI: 10.1016/j.foodres.2023.112615
Catarina Andrade ₁ , David M Pereira ₁ , Nelson G M Gomes ₁ , Federico Ferreres ₂ , Angel Gil-Izquierdo ₃ , Paula B Andrade ₁ , Sutsawat Duangsrisai ₄ , Patrícia Valentão ₁

Affiliation

Kitul ( Caryota urens L.) 花序在亚洲国家广泛用于生产甜汁，Kitul 食品被认为适合糖尿病患者。考虑到抑制 α-葡萄糖苷酶的强大能力，我们假设 kitul 的抗糖尿病特性可能还涉及调节炎症通路和高血糖引起的氧化损伤。因此，在葡萄糖刺激的胰腺细胞 (RIN-5F) 和 LPS 刺激的 RAW 264.7 巨噬细胞中研究了花序的甲醇提取物的作用。该提取物减少了胰腺细胞中细胞内活性物质的过量产生，也减少了 LPS 刺激的 RAW 264.7 巨噬细胞中的 NO、L-瓜氨酸和 IL-6 水平。抑制 5-脂氧合酶 (IC ₅₀= 166.1 µg/mL) 在用C. urens花序提取物处理时也记录了通过非竞争性方式。花序的酚类特征由 HPLC-DAD 表征，六种羟基肉桂酸被鉴定和量化。总的来说，我们的数据提供了关于 Kitul 花序作为抗糖尿病药物的多效机制的额外证据。

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Kitul, a food plant with antidiabetic-like effects: Reduction of intracellular reactive species in glucose-stimulated RIN-5F pancreatic β-cells and mitigation of pro-inflammatory mediators in activated RAW 264.7 macrophages

Kitul (Caryota urens L.) inflorescences are broadly used for sweet sap production in Asian countries and Kitul food products are known as being suitable for diabetic patients. Considering the strong ability to inhibit α-glucosidase, we hypothesize that kitul antidiabetic properties might also involve the modulation of inflammatory pathways and hyperglycaemia-induced oxidative damage. Hence, the effects of an inflorescence’s methanol extract were investigated in glucose-stimulated pancreatic cells (RIN-5F) and LPS-stimulated RAW 264.7 macrophages. The extract reduced the overproduction of intracellular reactive species in pancreatic cells and also NO, L-citrulline and IL-6 levels in LPS-stimulated RAW 264.7 macrophages. Inhibition of 5-lipoxygenase (IC₅₀ = 166.1 µg/mL) through an uncompetitive manner was also recorded upon treatment with C. urens inflorescences extract. The phenolic profile of the inflorescences was characterized by HPLC-DAD, six hydroxycinnamic acids being identified and quantified. Overall, our data provide additional evidence on the pleiotropic mechanisms of Kitul inflorescences as an antidiabetic agent.

更新日期：2023-02-24

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