评估益生菌戊糖片球菌 GS4 的 CLA 上调 PPARγ 在结肠癌缓解中的可能作用,PPAR Research

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评估益生菌戊糖片球菌 GS4 的 CLA 上调 PPARγ 在结肠癌缓解中的可能作用
PPAR Research ( IF 3.5 ) Pub Date : 2023-2-24 , DOI: 10.1155/2023/9458308
Vinay Dubey ₁ , Alok Kumar Mishra _{1,

2} , Asit Ranjan Ghosh ₁

Affiliation

结肠癌 (CC) 的患病率在流行范围内不断增加，伴随着随后的发病率和死亡率。尽管近年来在治疗策略方面取得了显著成就，但 CC 患者的治疗仍然是一项艰巨的任务。目前的研究重点是研究益生菌戊糖片球菌GS4 (CLA _GS4 ) 的生物氢化衍生共轭亚油酸 (CLA) 对 CC 的作用，从而诱导人 CC HCT-116 细胞中过氧化物酶体增殖物激活受体 γ (PPAR γ ) 的表达. PPARγ拮抗剂双酚A二缩水甘油醚预处理对HCT-116细胞增强细胞活力的抑制作用显着降低，提示PPARγ依赖性细胞死亡。_{用 CLA/CLA GS4}处理的癌细胞表现出与 COX-2 和 5-LOX 表达降低相关的前列腺素 E2 PGE ₂水平降低。此外，发现这些后果与 PPAR γ有关-依赖。此外，借助分子对接 LigPlot 分析线粒体依赖性细胞凋亡的描述表明，CLA 可以与己糖激酶-II (hHK-II)（在癌细胞中高表达）结合，并且这种关联是电压依赖性阴离子通道打开的基础，从而导致线粒体膜去极化，一种启动内在细胞凋亡事件的条件。通过膜联蛋白 V 染色和半胱天冬酶 1p10 表达的升高进一步证实细胞凋亡。综上所述，从机制上推断，P的CLA _{GS4对 PPAR}γ的上调。pentosaceus GS4 可以改变与触发 CC 细胞凋亡相关的癌细胞代谢。

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Appraisal of the Possible Role of PPARγ Upregulation by CLA of Probiotic Pediococcus pentosaceus GS4 in Colon Cancer Mitigation

The prevalence of colon cancer (CC) is increasing at the endemic scale, which is accompanied by subsequent morbidity and mortality. Although there have been noteworthy achievements in the therapeutic strategies in recent years, the treatment of patients with CC remains a formidable task. The current study focused on to study role of biohydrogenation-derived conjugated linoleic acid (CLA) of probiotic Pediococcus pentosaceus GS4 (CLA_GS4) against CC, which induced peroxisome proliferator-activated receptor gamma (PPARγ) expression in human CC HCT-116 cells. Pre-treatment with PPARγ antagonist bisphenol A diglycidyl ether has significantly reduced the inhibitory efficacy of enhanced cell viability of HCT-116 cells, suggesting the PPARγ-dependent cell death. The cancer cells treated with CLA/CLA_GS4 demonstrated the reduced level of Prostaglandin E2 PGE₂ in association with reduced COX-2 and 5-LOX expressions. Moreover, these consequences were found to be associated with PPARγ-dependent. Furthermore, delineation of mitochondrial dependent apoptosis with the help of molecular docking LigPlot analysis showed that CLA can bind with hexokinase-II (hHK-II) (highly expressed in cancer cells) and that this association underlies voltage dependent anionic channel to open, thereby causing mitochondrial membrane depolarization, a condition that initiates intrinsic apoptotic events. Apoptosis was further confirmed by annexin V staining and elevation of caspase 1p10 expression. Taken all together, it is deduced that, mechanistically, the upregulation of PPARγ by CLA_GS4 of P. pentosaceus GS4 can alter cancer cell metabolism in association with triggering apoptosis in CC.

更新日期：2023-02-24

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