For 2G ethanol production, pentose fermentation and yeast tolerance to lignocellulosic hydrolyzate components are essential to improve biorefinery yields. Generally, physicochemical pre-treatment methodologies are used to facilitate access to cellulose and hemicellulose in plant material, which consequently can generate microbial growth inhibitory compounds, such as furans, weak acids, and phenolic compounds. Because of the unsatisfactory yield of wild-type Saccharomyces cerevisiae during pentose fermentation, the search for xylose-fermenting yeasts tolerant to microbial growth inhibitors has gained attention. In this study, we investigated the ability of the yeasts Pichia guilliermondii G1.2 and Candida oleophila G10.1 to produce ethanol from xylose and tolerate the inhibitors furfural, 5-hydroxymethylfurfural (HMF), acetic acid, formic acid, ferulic acid, and vanillin. We demonstrated that both yeasts were able to grow and consume xylose in the presence of all single inhibitors, with greater growth limitation in media containing furfural, acetic acid, and vanillin. In saline medium containing a mixture of these inhibitors (2.5–3.5 mM furfural and HMF, 1 mM ferulic acid, 1–1.5 mM vanillin, 10–13 mM acetic acid, and 5–7 mM formic acid), both yeasts were able to produce ethanol from xylose, similar to that detected in the control medium (without inhibitors). In future studies, the proteins involved in the transport of pentose and tolerance to these inhibitors need to be investigated.

对于 2G 乙醇生产，戊糖发酵和酵母对木质纤维素水解产物成分的耐受性对于提高生物精炼产量至关重要。一般来说，物理化学预处理方法用于促进植物材料中纤维素和半纤维素的获取，从而产生微生物生长抑制化合物，例如呋喃、弱酸和酚类化合物。由于野生型酿酒酵母在戊糖发酵过程中产量不理想，寻找对微生物生长抑制剂具有耐受性的木糖发酵酵母引起了人们的关注。在这项研究中，我们研究了吉里蒙毕赤酵母G1.2 和产油假丝酵母G10.1 从木糖生产乙醇的能力以及对糠醛、5-羟甲基糠醛 (HMF)、乙酸、甲酸、阿魏酸和抑制剂的耐受性。香草醛。我们证明，两种酵母都能够在所有单一抑制剂存在的情况下生长和消耗木糖，在含有糠醛、乙酸和香草醛的培养基中具有更大的生长限制。在含有这些抑制剂混合物（2.5–3.5 mM 糠醛和 HMF、1 mM 阿魏酸、1–1.5 mM 香草醛、10–13 mM 乙酸和 5–7 mM 甲酸）的盐水培养基中，两种酵母都能够从木糖产生乙醇，与对照培养基（无抑制剂）中检测到的类似。在未来的研究中，需要研究参与戊糖转运的蛋白质以及对这些抑制剂的耐受性。