Intracellular Calcium Overload and Activation of CaMKK/AMPK Signaling Are Related to the Acceleration of Muscle Glycolysis of Broiler Chickens Subjected to Acute Stress,Journal of Agricultural and Food Chemistry

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Intracellular Calcium Overload and Activation of CaMKK/AMPK Signaling Are Related to the Acceleration of Muscle Glycolysis of Broiler Chickens Subjected to Acute Stress
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-02-23 , DOI: 10.1021/acs.jafc.2c07391
Hongju Liao ₁ , Lin Zhang ₁ , Jiaolong Li ₂ , Tong Xing ₁ , Feng Gao ₁

Affiliation

The current study investigated the effect of preslaughter transport on stress response and meat quality of broilers and explored the underlying mechanisms involved in the regulation of muscle glycolysis through calcium/calmodulin-dependent protein kinase kinase (CaMKK)/AMP-activated protein kinase (AMPK) signaling. Results suggested that transport induced stress responses of broilers and caused PSE-like syndrome of pectoralis major muscle. Preslaughter transport enhanced the mRNA expressions of glycogen phosphorylase and glucose transporters, as well as the activities of glycolytic enzymes, which accelerated the breakdown of glycolytic substrates and the accumulation of lactic acid. In addition, acute stress induced abnormal intracellular calcium homeostasis by disrupting calcium channels on the cell membrane and sarcoplasmic reticulum, which led to the activation of CaMKK and promoted AMPK phosphorylation. This study provides evidence that the intracellular calcium overload and the enhancement of CaMKK/AMPK signaling are related to the accelerated muscle glycolysis of broiler chickens subjected to acute stress.

中文翻译：

细胞内钙超载和 CaMKK/AMPK 信号通路的激活与急性应激下肉鸡肌肉糖酵解的加速有关

目前的研究调查了屠宰前运输对肉鸡应激反应和肉质的影响，并探索了通过钙/钙调蛋白依赖性蛋白激酶激酶 (CaMKK)/AMP 活化蛋白激酶 (AMPK) 调节肌肉糖酵解的潜在机制信号。结果表明，运输诱导了肉鸡的应激反应，并引起了胸大肌的 PSE 样综合征。屠宰前转运增强了糖原磷酸化酶和葡萄糖转运蛋白的 mRNA 表达，以及糖酵解酶的活性，加速了糖酵解底物的分解和乳酸的积累。此外，急性应激通过破坏细胞膜和肌浆网上的钙通道诱导细胞内钙稳态异常，从而导致 CaMKK 的激活并促进 AMPK 磷酸化。本研究提供的证据表明，细胞内钙过载和 CaMKK/AMPK 信号增强与肉鸡在急性应激下的肌肉糖酵解加速有关。

更新日期：2023-02-23

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