脂质体和脂质纳米颗粒的形态学行为,Langmuir

当前位置： X-MOL 学术 › Langmuir › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

脂质体和脂质纳米颗粒的形态学行为
Langmuir ( IF 3.7 ) Pub Date : 2023-02-22 , DOI: 10.1021/acs.langmuir.2c02794
Igor V Zhigaltsev ₁ , Pieter R Cullis ₁

Affiliation

脂质体由围绕内部水性隔室的双层脂质组成，大约在 60 年前首次被表征。值得注意的是，脂质体及其胶束状“实心核”对应物（疏水核周围的脂质单层）的许多基本特性以及这些结构之间的转变仍然知之甚少。在这项工作中，我们检查了基本变量对基于脂质的系统所采用的形态的影响，该系统通过将乙醇中的脂质与水性介质快速混合而产生。我们表明，对于在水合作用时形成双层囊泡的双硬脂酰磷脂酰胆碱 (DSPC)-胆固醇混合物等脂质，渗透压可诱导高正膜曲率区域，导致单层囊泡之间融合产生双层囊泡。添加溶血PC，支持高正曲率区域的“倒锥”形脂质可以通过稳定半融合中间结构来抑制这些双层囊泡的形成。相反，导致膜负曲率的“锥”形脂质如二油酰磷脂酰乙醇胺 (DOPE) 的存在会促进囊泡形成后的融合事件（在乙醇透析阶段），即使在没有渗透压的情况下也会导致双层和多层系统压力。或者，存在增加量的三油精（一种不溶于脂质双层的脂质）会导致内部固体核心结构增加，直到获得具有三油精疏水核心的胶束样系统。这些结果根据双层囊泡可以稳定保持的内在膜曲率以及双层脂质首先在疏水材料（如三油精）的固体核心周围形成单层的能力来解释，然后，双层脂质的比例是增加，逐渐形成双层结构，最终可以形成一个完整的双层结构，将疏水核和水性隔室包裹起来。这些混合中间结构可用作新型药物输送系统。逐渐形成双层结构，最终可以形成一个完整的双层结构，将疏水核和水性隔室包裹起来。这些混合中间结构可用作新型药物输送系统。逐渐形成双层结构，最终可以形成一个完整的双层结构，将疏水核和水性隔室包裹起来。这些混合中间结构可用作新型药物输送系统。

"点击查看英文标题和摘要"

Morphological Behavior of Liposomes and Lipid Nanoparticles

Liposomes, which consist of bilayer lipids surrounding interior aqueous compartment(s), were first characterized nearly 60 years ago. Remarkably, many fundamental properties of liposomes and their micellar-like “solid core” counterparts (a lipid monolayer surrounding a hydrophobic core) and transitions between these structures remain poorly understood. In this work, we examine the effects of basic variables on the morphology adopted by lipid-based systems produced by rapid mixing of lipids in ethanol with aqueous media. We show that, for lipids such as distearolyphosphatidylcholine (DSPC)–cholesterol mixtures that form bilayer vesicles on hydration, osmotic stress can induce regions of high positive membrane curvature, leading to fusion between unilamellar vesicles to produce bilamellar vesicles. Addition of lyso PC, an “inverted cone”-shaped lipid that supports regions of high positive curvature, can inhibit the formation of these bilamellar vesicles by stabilizing a hemifused intermediate structure. Conversely, the presence of “cone”-shaped lipids such as dioleoylphosphatidylethanolamine (DOPE) that results in negative membrane curvature promotes fusion events subsequent to vesicle formation (during the ethanol dialysis stage), leading to bilamellar and multilamellar systems even in the absence of osmotic stress. Alternatively, the presence of increasing amounts of triolein, a lipid that is insoluble in lipid bilayers, results in increasing internal solid core structures until micellar-like systems with a hydrophobic core of triolein are achieved. These results are interpreted in terms of the intrinsic membrane curvature that bilayer vesicles can stably maintain as well as the ability of bilayer lipids to first form a monolayer around a solid core of hydrophobic material such as triolein and then, as the proportion of bilayer lipids is increased, progressively form bilayer structures that can eventually form a complete bilayer encapsulating both a hydrophobic core and an aqueous compartment. These hybrid intermediate structures may have utility as novel drug delivery systems.

更新日期：2023-02-22

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南