室温动态顶空固相萃取：异丙酚分析的理论模型、方法和应用,Analytical Methods

当前位置： X-MOL 学术 › Anal. Methods › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

室温动态顶空固相萃取：异丙酚分析的理论模型、方法和应用
Analytical Methods ( IF 2.7 ) Pub Date : 2023-02-21 , DOI: 10.1039/d2ay02099h
Sarah Nakhodchi ₁ , Naader Alizadeh ₁

Affiliation

在此，在室温下使用简单的动态顶空固相萃取 (DHS-SPE) 工艺处理对温度升高敏感的材料。实施了一种拟议的方法，在荧光光谱分析之前从复杂基质中快速提取异丙酚 (PF)，在短采样时间内无需使用热板或搅拌器。使用微型隔膜泵循环顶部空间气体。当顶部空间气体流过样品溶液表面时，气泡形成并将分析物从液体中释放到顶部空间中。在提取过程中，顶部空间气体作为吸附剂穿过带涂层的金属泡沫，吸附剂置于自制玻璃容器中，分析物从气相中捕获。本研究提出了一种基于连续一阶过程的 DHS-SPE 理论模型。通过将顶空和吸附器中分析物浓度的变化与泵速和提取到固相的分析物量相关联，获得了传质动态过程的数学解。使用泡沫镍上的电化学掺杂 Nafion 的聚吡咯 (PPy-Naf) 薄膜作为固相耦合荧光检测，获得了 100-500 nM 浓度范围内的线性动态范围，检测限为 15 nM。该方法成功应用于人血清样品基质中的 PF 测定，而不受共同给药药物（如具有显着发射光谱重叠的顺式阿曲库铵）的干扰。所开发的方法可以为样品预处理带来新思路，它与许多分析技术兼容，并在这项工作中成功地与荧光光谱相结合。这种采样形式简化了分析物从复杂基质到顶空的萃取和预浓缩过程的转移，消除了加热步骤和对昂贵设备的需求。

"点击查看英文标题和摘要"

Dynamic headspace solid-phase extraction at room temperature: a theoretical model, method, and application for propofol analysis

Herein, a simple dynamic headspace solid-phase extraction (DHS-SPE) process at room temperature was used for a material that is sensitive to increase in the temperature. A proposed method was implemented to rapidly extract propofol (PF) from a complex matrix before fluorescence spectroscopy analysis, within a short sampling time without involving a hot plate or stirrer. A mini diaphragm pump was used to circulate the headspace gas. As the headspace gas flows over the sample solution surface, bubbles form and release analytes from the liquid into the headspace. During the extraction process, the headspace gas passes through the coated metal foam as a sorbent that is placed in a homemade glass vessel and analytes are trapped from the gas phase. A theoretical model of DHS-SPE based on the consecutive first-order process is proposed in this study. A mathematical solution for the dynamic process of mass transfer was obtained by correlating the variation in analyte concentration in the headspace and adsorber with the pump speed and amount of analyte extracted to the solid phase. Using electrochemically Nafion-doped polypyrrole (PPy-Naf) film on nickel foam as the solid-phase coupled to fluorescence detection, a linear dynamic range over the concentration range of 100–500 nM with a detection limit of 15 nM was obtained. This method was applied successfully for PF determination in human serum sample matrices without the interference of co-administered drugs, such as cisatracurium, which have significant emission spectrum overlap. The developed method can lead to a new idea for sample pretreatment, which is compatible with many analytical techniques and has been successfully combined with fluorescence spectroscopy in this work. This format of sampling simplifies the transfer of analytes from complex matrices to the headspace for the extraction and preconcentration process, eliminating the heating step and the need for expensive equipment.

更新日期：2023-02-21

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南