DNA methyltransferases (MTases) can establish various epigenetic modification patterns through catalyzing methylation of adenine/cytosine DNA bases. Deregulation of DNA MTases activity may lead to aberrant DNA methylation patterns and eventually induces different diseases. DNA MTases have become valuable targets for drug screening and disease therapy, and efficient detection of DNA MTase activity is critical to epigenetic researches and clinical diagnostics. Recently, various nucleic acid amplification strategies have been exploited for sensitively sensing low-abundance DNA MTase. Herein, we review the advance in the development of nucleic acid amplification-integrated methods for DNA methyltransferase assay. Nucleic acid amplification can be achieved by using protein enzymes (e.g., DNA/RNA polymerases and DNA/RNA nucleases), enzyme-free strategies (e.g., DNAzyme, entropy-driven amplification, hybridization chain reaction, and catalytic hairpin assembly), and their combinations. This review focuses on the principles, features, and applications of nucleic acid amplification-integrated methods, and highlight the challenges and future direction in the field as well.

DNA 甲基转移酶 (MTases) 可以通过催化腺嘌呤/胞嘧啶 DNA 碱基的甲基化来建立各种表观遗传修饰模式。DNA 转移酶活性的失调可能导致异常的 DNA 甲基化模式，并最终诱发不同的疾病。DNA MTase 已成为药物筛选和疾病治疗的重要靶标，有效检测 DNA MTase 活性对于表观遗传学研究和临床诊断至关重要。最近，各种核酸扩增策略已被用于灵敏检测低丰度 DNA 转移酶。在此，我们综述了用于DNA甲基转移酶测定的核酸扩增整合方法的发展进展。可以通过使用蛋白质酶（例如，DNA/RNA聚合酶和DNA/RNA核酸酶）实现核酸扩增，无酶策略（例如，DNAzyme、熵驱动扩增、杂交链式反应和催化发夹组装）及其组合。本综述重点介绍了核酸扩增一体化方法的原理、特点和应用，并重点介绍了该领域面临的挑战和未来发展方向。