具有快速内部组织浸润和血管化能力的花坛仿生支架用于骨修复,ACS Nano

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具有快速内部组织浸润和血管化能力的花坛仿生支架用于骨修复
ACS Nano ( IF 15.8 ) Pub Date : 2023-02-21 , DOI: 10.1021/acsnano.3c00598
Xiaojun Zhou ₁ , Yuhan Qian ₁ , Liang Chen ₂ , Tao Li ₃ , Xin Sun ₃ , Xiaojun Ma ₄ , Jinwu Wang ₃ , Chuanglong He ₁

Affiliation

组织工程骨支架的良好微观结构和生物活性与骨缺损的再生功效密切相关。然而，对于大骨缺损的治疗，它们大多不能满足足够的机械强度、高度多孔的结构以及优异的血管生成和成骨活性等要求。在此，受“花坛”特征的启发，我们构建了一种富含短纳米纤维聚集体的双因子递送支架，通过用于引导血管化骨再生的 3D 打印和静电纺丝技术。通过将含有二甲基草酰甘氨酸 (DMOG) 负载的介孔二氧化硅纳米粒子的短纳米纤维与 3D 打印的含锶羟基磷灰石/聚己内酯 (SrHA@PCL) 支架组装在一起，可以通过改变纳米纤维的密度轻松实现可调节的多孔结构，同时坚固由于 SrHA@PCL 的框架作用，将获得抗压强度。由于电纺纳米纤维和 3D 打印微丝之间的不同降解性能，实现了 DMOG 和 Sr 离子的顺序释放行为。体内和体外_结果表明，双因子递送支架具有良好的生物相容性，通过刺激内皮细胞和成骨细胞显着促进血管生成和成骨，并通过激活缺氧诱导因子-1α通路和免疫调节作用有效加速组织向内生长和血管化骨再生。总的来说，这项研究为构建用于骨再生的骨微环境匹配的仿生支架提供了一种有前途的策略。

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Flowerbed-Inspired Biomimetic Scaffold with Rapid Internal Tissue Infiltration and Vascularization Capacity for Bone Repair

The favorable microstructure and bioactivity of tissue-engineered bone scaffolds are closely associated with the regenerative efficacy of bone defects. For the treatment of large bone defects, however, most of them fail to meet requirements such as adequate mechanical strength, highly porous structure, and excellent angiogenic and osteogenic activities. Herein, inspired by the characteristics of a “flowerbed”, we construct a short nanofiber aggregates-enriched dual-factor delivery scaffold via 3D printing and electrospinning techniques for guiding vascularized bone regeneration. By the assembly of short nanofibers containing dimethyloxalylglycine (DMOG)-loaded mesoporous silica nanoparticles with a 3D printed strontium-contained hydroxyapatite/polycaprolactone (SrHA@PCL) scaffold, an adjustable porous structure can be easily realized by changing the density of nanofibers, while strong compressive strength will be acquired due to the framework role of SrHA@PCL. Owing to the different degradation performance between electrospun nanofibers and 3D printed microfilaments, a sequential release behavior of DMOG and Sr ions is achieved. Both in vivo and in vitro results demonstrate that the dual-factor delivery scaffold has excellent biocompatibility, significantly promotes angiogenesis and osteogenesis by stimulating endothelial cells and osteoblasts, and effectively accelerates tissue ingrowth and vascularized bone regeneration through activating the hypoxia inducible factor-1α pathway and immunoregulatory effect. Overall, this study has provided a promising strategy for constructing a bone microenvironment-matched biomimetic scaffold for bone regeneration.

更新日期：2023-02-21

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