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Molecular biological features of cyst wall of adamantinomatous craniopharyngioma
Scientific Reports ( IF 3.8 ) Pub Date : 2023-02-21 , DOI: 10.1038/s41598-023-29664-z
Chuan Zhao 1, 2 , Ye Wang 3 , Hongxing Liu 1, 4 , Xueling Qi 5 , Zhongqing Zhou 1 , Xianlong Wang 6 , Zhixiong Lin 1
Affiliation  

The molecular biological differences between cyst walls and those in solid bodies are the foundation of the outcomes. In this study, the CTNNB1 mutations were confirmed by DNAsequencing; CTNNB1 expression levels were detected by PCR; the differences between solid bodies and cyst walls in proliferative capacity and tumor stem cell niches were assessed by immunohistochemistry; the effect of the residual cyst wall on recurrence was assessed by follow-up. Mutations in the CTNNB1 in the cyst wall and the solid body were identical in each case. No differences were found in the transcriptional level of CTNNB1 between the cyst walls and the solid bodies (P = 0.7619). The cyst wall showed a pathological structure similar to the solid body. Proliferative capacity of cyst walls was stronger than that of solid body (P = 0.0021), and β-catenin nuclear positive cells (cell clusters) in cyst walls were more than that in solid tumor (P = 0.0002). The retrospective 45 ACPs showed residual cyst wall was significantly associated with tumor recurrence or regrowth (P = 0.0176). Kaplan–Meier analysis showed there was a significant difference in the prognosis between GTR and STR (P < 0.0001).The cyst wall of ACP contained more tumor stem cell niches which could lead to the recurrence. According to the above-mentioned, a special attention to the management of the cyst wall should be paid.



中文翻译:

坚固型颅咽管瘤囊壁的分子生物学特征

囊肿壁与实体中的囊肿壁之间的分子生物学差异是结果的基础。在这项研究中,CTNNB1突变通过 DNA 测序得到证实;PCR检测CTNNB1表达水平;通过免疫组织化学评估实体和囊肿壁在增殖能力和肿瘤干细胞生态位方面的差异;通过随访评估残余囊壁对复发的影响。包囊壁和实体中的CTNNB1突变在每种情况下都是相同的。CTNNB1的转录水平在包囊壁和实体之间没有发现差异( P = 0.7619)。囊壁呈与实性体相似的病理结构。囊壁增殖能力强于实体瘤(P  =0.0021),囊壁β-catenin核阳性细胞(细胞团)多于实体瘤(P  =0.0002)。回顾性 45 个 ACPs 显示残余囊壁与肿瘤复发或再生长显着相关(P  = 0.0176)。Kaplan-Meier分析显示GTR与STR的预后存在显着差异(P  < 0.0001)。ACP的囊壁含有较多的肿瘤干细胞巢,可导致复发。综上所述,应特别注意囊壁的处理。

更新日期:2023-02-21
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