A Photoinduced Electron Transfer-Based Hypochlorite-Specific Fluorescent Probe for Selective Imaging of Proinflammatory M1 in a Rheumatoid Arthritis Model,Analytical Chemistry

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A Photoinduced Electron Transfer-Based Hypochlorite-Specific Fluorescent Probe for Selective Imaging of Proinflammatory M1 in a Rheumatoid Arthritis Model
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-02-17 , DOI: 10.1021/acs.analchem.2c05218
Mousumi Baruah ₁ , Haw-Young Kwon ₂ , Heewon Cho ₃ , Young-Tae Chang _{2,

4} , Animesh Samanta ₁

Affiliation

The differentiation of the distinct phenotypes of macrophages is essential for monitoring the stage of inflammatory diseases for accurate diagnosis and treatment. Recent studies revealed that the level of hypochlorite (OCl^–) varies from activated M1 macrophages (killing pathogens) to M2 (resolution of inflammation) during inflammation. Thus, we developed a simple and efficient fluorescent probe for discriminating M1 from M0 and M2. Herein, fluorescent-based imaging is applied as an alternative to immunohistochemistry, which is challenging due to the tedious process and high cost. We developed a hypochlorite-specific probe PMS-T to differentiate M1 and M2, employing a metabolism-oriented live-cell distinction. This probe enables the detection of inflammatory rheumatoid arthritis in an ex vivo mouse model. Thus, it can be a potential chemical tool for monitoring inflammatory diseases, including rheumatoid arthritis, that may overcome the existing barriers of immunohistochemistry.

中文翻译：

一种基于光诱导电子转移的次氯酸盐特异性荧光探针，用于类风湿性关节炎模型中促炎性 M1 的选择性成像

巨噬细胞不同表型的分化对于监测炎症性疾病的阶段以进行准确诊断和治疗至关重要。^{最近的研究表明，次氯酸盐 (OCl –} )的水平在炎症期间从激活的 M1 巨噬细胞（杀死病原体）到 M2（消退炎症）不等。因此，我们开发了一种简单有效的荧光探针，用于区分 M1 与 M0 和 M2。在此，基于荧光的成像被用作免疫组织化学的替代方法，由于繁琐的过程和高成本，免疫组织化学具有挑战性。我们开发了一种次氯酸盐特异性探针PMS-T来区分 M1 和 M2，采用以代谢为导向的活细胞区分。该探针能够检测炎性类风湿性关节炎离体小鼠模型。因此，它可以成为监测炎症性疾病（包括类风湿性关节炎）的潜在化学工具，可以克服免疫组织化学的现有障碍。

更新日期：2023-02-17

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