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Orange-Emissive Carbon Dots with High Photostability for Mitochondrial Dynamics Tracking in Living Cells
ACS Sensors ( IF 8.2 ) Pub Date : 2023-02-16 , DOI: 10.1021/acssensors.2c02451 Nini Xin 1 , Dong Gao 1 , Borui Su 1 , Ting Zhou 1 , Yuda Zhu 2 , Chengheng Wu 1, 3 , Dan Wei 1 , Jing Sun 1 , Hongsong Fan 1
ACS Sensors ( IF 8.2 ) Pub Date : 2023-02-16 , DOI: 10.1021/acssensors.2c02451 Nini Xin 1 , Dong Gao 1 , Borui Su 1 , Ting Zhou 1 , Yuda Zhu 2 , Chengheng Wu 1, 3 , Dan Wei 1 , Jing Sun 1 , Hongsong Fan 1
Affiliation
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Mitochondria play significant roles in maintaining a stable internal environment for cell metabolism. Hence, real-time monitoring of the dynamics of mitochondria is essential for further understanding mitochondria-related diseases. Fluorescent probes provide powerful tools for visualizing dynamic processes. However, most mitochondria-targeted probes are derived from organic molecules with poor photostability, making long-term dynamic monitoring challenging. Herein, we design a novel mitochondria-targeted probe based on carbon dots with high performance for long-term tracking. Considering that the targeting ability of CDs is related to surface functional groups, which are generally determined by the reaction precursors, we successfully constructed mitochondria-targeted O-CDs with emission at 565 nm through solvothermal treatment of m-diethylaminophenol. The O-CDs are bright with a high quantum yield of 12.61%, high mitochondria-targeting ability, and good stability. The O-CDs possess a high quantum yield (12.61%), specific mitochondria-targeting ability, and outstanding optical stability. Owing to the abundant hydroxyl and ammonium cations on the surface, O-CDs showed obvious accumulation in mitochondria with a high colocalization coefficient of up to 0.90 and remained steady even after fixation. Besides, O-CDs showed outstanding compatibility and photostability under various interruptions or long-time irradiation. Therefore, O-CDs are preferable for the long-term tracking of dynamic mitochondrial behavior in live cells. We first observed the mitochondrial fission and fusion behaviors in HeLa cells, and then, the size, morphology, and distribution of mitochondria in physiological or pathological conditions were clearly recorded. More importantly, we observed different dynamics interactions between mitochondria and lipid droplets during the apoptosis and mitophagy processes. This study provides a potential tool for exploring interactions between mitochondria and other organelles, further promoting the research on mitochondria-related diseases.
中文翻译：
具有高光稳定性的橙色发射碳点用于活细胞中的线粒体动力学跟踪
线粒体在维持细胞代谢稳定的内部环境中起着重要作用。因此，实时监测线粒体动力学对于进一步了解线粒体相关疾病至关重要。荧光探针为可视化动态过程提供了强大的工具。然而，大多数线粒体靶向探针来源于光稳定性差的有机分子，使得长期动态监测具有挑战性。在此，我们设计了一种基于碳点的新型线粒体靶向探针，具有长期跟踪的高性能。考虑到 CDs 的靶向能力与表面官能团有关，而表面官能团通常由反应前体决定，我们通过溶剂热处理成功构建了线粒体靶向 O-CDs，发射波长为 565 nm米-二乙基氨基苯酚。O-CDs光亮，量子产率高达12.61%，线粒体靶向能力强，稳定性好。O-CD 具有高量子产率 (12.61%)、特定的线粒体靶向能力和出色的光学稳定性。由于表面存在丰富的羟基和铵阳离子，O-CDs 在线粒体中有明显的积累，共定位系数高达 0.90，即使在固定后也能保持稳定。此外，O-CD在各种中断或长时间照射下表现出出色的相容性和光稳定性。因此，O-CD 更适合长期跟踪活细胞中的动态线粒体行为。我们首先观察了 HeLa 细胞中线粒体的分裂和融合行为，然后是大小、形态、清楚地记录了线粒体在生理或病理条件下的分布情况。更重要的是，我们观察到了细胞凋亡和线粒体自噬过程中线粒体和脂滴之间不同的动力学相互作用。该研究为探索线粒体与其他细胞器之间的相互作用提供了一个潜在的工具，进一步推动了线粒体相关疾病的研究。
更新日期：2023-02-16
中文翻译：
具有高光稳定性的橙色发射碳点用于活细胞中的线粒体动力学跟踪
线粒体在维持细胞代谢稳定的内部环境中起着重要作用。因此，实时监测线粒体动力学对于进一步了解线粒体相关疾病至关重要。荧光探针为可视化动态过程提供了强大的工具。然而，大多数线粒体靶向探针来源于光稳定性差的有机分子，使得长期动态监测具有挑战性。在此，我们设计了一种基于碳点的新型线粒体靶向探针，具有长期跟踪的高性能。考虑到 CDs 的靶向能力与表面官能团有关，而表面官能团通常由反应前体决定，我们通过溶剂热处理成功构建了线粒体靶向 O-CDs，发射波长为 565 nm米-二乙基氨基苯酚。O-CDs光亮，量子产率高达12.61%，线粒体靶向能力强，稳定性好。O-CD 具有高量子产率 (12.61%)、特定的线粒体靶向能力和出色的光学稳定性。由于表面存在丰富的羟基和铵阳离子，O-CDs 在线粒体中有明显的积累，共定位系数高达 0.90，即使在固定后也能保持稳定。此外，O-CD在各种中断或长时间照射下表现出出色的相容性和光稳定性。因此，O-CD 更适合长期跟踪活细胞中的动态线粒体行为。我们首先观察了 HeLa 细胞中线粒体的分裂和融合行为，然后是大小、形态、清楚地记录了线粒体在生理或病理条件下的分布情况。更重要的是，我们观察到了细胞凋亡和线粒体自噬过程中线粒体和脂滴之间不同的动力学相互作用。该研究为探索线粒体与其他细胞器之间的相互作用提供了一个潜在的工具，进一步推动了线粒体相关疾病的研究。
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