载有葡萄糖氧化酶的 MnFe2O4 纳米颗粒用于热疗和癌症饥饿疗法,ACS Applied Nano Materials

当前位置： X-MOL 学术 › ACS Appl. Nano Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

载有葡萄糖氧化酶的 MnFe2O4 纳米颗粒用于热疗和癌症饥饿疗法
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2023-02-16 , DOI: 10.1021/acsanm.2c04960
Monunith Anithkumar _{1,

2} , Arunima Rajan S _{1,

3} , Ahmaduddin Khan ₁ , Beata Kaczmarek ₄ , Marta Michalska-Sionkowska ₅ , Krzysztof Łukowicz ₆ , Anna Maria Osyczka ₆ , Jagriti Gupta ₇ , Niroj Kumar Sahu ₁

Affiliation

纳米粒子介导的饥饿疗法是一种很有前途的癌症治疗方法。在这里，我们报告了一种新型治疗性纳米系统 (GOx@PEG-MnFe ₂ O ₄ )，由负载在聚乙二醇 (PEG) 改性锰铁氧体 (MnFe ₂ O ₄ ) 纳米颗粒 (NPs) 中的葡萄糖氧化酶 (GOx) 组成，用于癌症饥饿治疗。GOx在酸性环境中通过消耗氧气 (O ₂ )催化葡萄糖氧化为葡萄糖酸和过氧化氢 (H ₂ O _{2 )。}同时，NPs可以催化H ₂ O ₂氧化生成O ₂，这反过来又有助于消耗葡萄糖并消除缺氧状况。NP 的相鉴定、晶体结构和尺寸证实了立方尖晶石结构，平均微晶尺寸为 21 nm。NPs 表现出良好的磁化率，室温下的磁化强度约为 75 emu/g。获得的比吸收率 (SAR) 为 296 W/g 的 NPs 的加热潜力证明了 NPs 作为癌症热疗加热剂的功效，这有助于将磁热疗与基于葡萄糖氧化酶的饥饿疗法相结合的协同疗法. 傅里叶变换红外光谱和热重分析证实 GOx 成功加载到 PEG-MnFe ₂ O _4上纳米系统。在应用磁场中，纳米系统在癌细胞模拟环境中释放出约 82% 的 GOx，比正常细胞模拟环境高约 1.8 倍。针对 HeLa 和 Saos-2 癌细胞系的体外评估显示出抗癌活性并表现出降低的溶血率。此外，体外结果表明 NPs 具有生物相容性并具有潜在的高温能力。设计的纳米系统能够通过癌症饥饿疗法实现杀瘤作用。

"点击查看英文标题和摘要"

Glucose Oxidase-Loaded MnFe2O4 Nanoparticles for Hyperthermia and Cancer Starvation Therapy

Nanoparticle-mediated starvation therapy is a promising therapeutic approach for cancer treatment. Here, we report a novel therapeutic nanosystem (GOx@PEG-MnFe₂O₄) composed of glucose oxidase (GOx) loaded in polyethylene glycol (PEG) modified manganese ferrite (MnFe₂O₄) nanoparticles (NPs) for cancer starvation therapy. GOx catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide (H₂O₂) by consuming oxygen (O₂) in an acidic environment. Meanwhile, NPs can catalyze the oxidation of H₂O₂ to generate O₂, which, in turn, aids in the depletion of glucose and eliminate the hypoxia condition. Phase identification, crystal structure, and size of the NPs confirm the cubic spinel structure with an average crystallite size of 21 nm. NPs exhibit good magnetic susceptibility with a magnetization value of ∼75 emu/g at room temperature. Heating potentiality of the NPs with an obtained specific absorption rate (SAR) of 296 W/g proves the efficacy of NPs to act as a heating agent for cancer hyperthermia, which aid for a synergistic therapy combining magnetic hyperthermia with glucose oxidase-based starvation therapy. Fourier transform infrared spectroscopy and thermogravimetry analysis confirm the successful loading of GOx onto the PEG-MnFe₂O₄ nanosystem. The nanosystem exhibited ∼82% release of GOx in the cancer cell-mimicking environment in an applied magnetic field which is around 1.8 times higher than that in the normal cell-mimicking environment. In vitro assessment against HeLa and Saos-2 cancer cell lines demonstrated anticancer activity and exhibited reduced hemolysis rates. Furthermore, the in vitro results suggested that the NPs are biocompatible and have potential hyperthermic ability. The designed nanosystem is capable of effectuating the tumoricidal effect via cancer starvation therapy.

更新日期：2023-02-16

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南